| Antigen detectable with this ELISA kit: |
Cyclin Dependent Kinase Inhibitor 2A (CDKN2A) |
| Species reactivity: |
Mus musculus (Mouse) |
| Range of detection: |
0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity: |
108ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Tissue homogenates and other biological fluids |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cyclin Dependent Kinase Inhibitor 2A (CDKN2A), The concentration of Cyclin Dependent Kinase Inhibitor 2A (CDKN2A) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin Dependent Kinase Inhibitor 2A (CDKN2A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Cyclin Dependent Kinase Inhibitor 2A (CDKN2A), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Description: |
Not all receptor antagonist that bind to enzymes are inhibitors, Since blocking an enzyme's activity can kill a pathogen , activator, caspase, esterase inhibitors , lipoprotein, many drugs are enzyme inhibitors, papain, pathway, peptidase, protease , proteinase, trypsin, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme, , Tissue, acrosin, activity, and decreases its , are , bind to enzymes and increase their , enzymatic activity, enzyme activator ligands or agonists , enzyme receptor , imbalance, metabolic , or correct a , or receptor ligands or receptor antagonists that bind to an , proteins |
| Gene target: |
Cyclin Dependent Kinase Inhibitor 2A (CDKN2A) |
| Short name: |
Cyclin Dependent Kinase Inhibitor 2A (CDKN2A) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Cyclin Dependent phosphorylation catalyst suppressor 2A (cyclin-dependent kinase inhibitor 2A) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
ARF and CDK4I and CDKN2 and CMM2 and INK4 and INK4A and MLM and MTS-1 and MTS1 and P14 and P14ARF and P16 and P16-INK4A and P16INK4 and P16INK4A and P19 and P19ARF and TP16, CDKN2A and IDBG-54547 and ENSG00000147889 and 1029, CDKN2A and IDBG-630043 and ENSBTAG00000034220 and 616369, DNA-templated and biological process this GO :0006364 and rRNA processing and biological process this GO :0006469 and negative regulation of protein kinase activity and biological process this GO :0006919 and activation of cysteine-type endopeptidase activity involved in apoptotic process and biological process this GO :0007050 and cell cycle arrest and biological process this GO :0007265 and Ras protein signal transduction and biological process this GO :0008134 and transcription factor binding and molecular function this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0008637 and apoptotic mitochondrial changes and biological process this GO :0010389 and regulation of G2/M transition of mitotic cell cycle and biological process this GO :0016301 and kinase activity and molecular function this GO :0016310 and phosphorylation and biological process this GO :0016604 and nuclear body and cellular component this GO :0019901 and protein kinase binding and molecular function this GO :0030308 and negative regulation of cell growth and biological process this GO :0030889 and negative regulation of B cell proliferation and biological process this GO :0031647 and regulation of protein stability and biological process this GO :0031648 and protein destabilization and biological process this GO :0032088 and negative regulation of NF-kappaB transcription factor activity and biological process this GO :0033088 and negative regulation of immature T cell proliferation in thymus and biological process this GO :0033235 and positive regulation of protein sumoylation and biological process this GO :0034393 and positive regulation of smooth muscle cell apoptotic process and biological process this GO :0035985 and senescence-associated heterochromatin focus and cellular component this GO :0035986 and senescence-associated heterochromatin focus assembly and biological process this GO :0042326 and negative regulation of phosphorylation and biological process this GO :0043234 and protein complex and cellular component this GO :0043517 and positive regulation of DNA damage response, DNA-templated and biological process this GO :0045893 and positive regulation of transcription, DNA-templated and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0046825 and regulation of protein export from nucleus and biological process this GO :0048103 and somatic stem cell division and biological process this GO :0050821 and protein stabilization and biological process this GO :0051059 and NF-kappaB binding and molecular function this GO :0051444 and negative regulation of ubiquitin-protein transferase activity and biological process this GO :0055105 and ubiquitin-protein transferase inhibitor activity and molecular function this GO :0070534 and protein K63-linked ubiquitination and biological process this GO :0071158 and positive regulation of cell cycle arrest and biological process this GO :0090398 and cellular senescence and biological process this GO :0090399 and replicative senescence and biological process this GO :0097371 and MDM2/MDM4 family protein binding and molecular function this GO :1902510 and regulation of apoptotic DNA fragmentation and biological process this GO :2000111 and positive regulation of macrophage apoptotic process and biological process this GO :2000774 and positive regulation of cellular senescence and biological process, MDM2/MDM4 family protein binding, nuclei, signal transduction by p53 class mediator and biological process this GO :0044822 and poly(A) RNA binding and molecular function this GO :0045087 and innate immune response and biological process this GO :0045736 and negative regulation of cyclin-dependent protein serine/threonine kinase activity and biological process this GO :0045892 and negative regulation of transcription, this GO :0000075 and cell cycle checkpoint and biological process this GO :0000082 and G1/S transition of mitotic cell cycle and biological process this GO :0000209 and protein polyubiquitination and biological process this GO :0000278 and mitotic cell cycle and biological process this GO :0001953 and negative regulation of cell-matrix adhesion and biological process this GO :0002039 and p53 binding and molecular function this GO :0003677 and DNA binding and molecular function this GO :0004861 and cyclin-dependent protein serine/threonine kinase inhibitor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005730 and nucleolus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005739 and mitochondrion and cellular component this GO :0005829 and cytosol and cellular component this GO :0006351 and transcription, this GO :0002039 : p53 binding, this GO :0002039 : p53 binding and also this GO :0003677 : DNA binding and also this GO :0004861 : cyclin-dependent protein serine/threonine kinase inhibitor activity and also this GO :0005515 : protein binding and also this GO :0008134 : transcription factor binding and also this GO :0016301 : kinase activity and also this GO :0019901 : protein kinase binding and also this GO :0044822 : poly(A) RNA binding and also this GO :0051059 : NF-kappaB binding and also this GO :0055105 : ubiquitin-protein transferase inhibitor activity and also this GO :0097371 : MDM2/MDM4 family protein binding, this GO :0003677 : DNA binding, this GO :0004861 : cyclin-dependent protein serine/threonine kinase inhibitor activity, this GO :0005515 : protein binding, this GO :0008134 : transcription factor binding, this GO :0016301 : kinase activity, this GO :0019901 : protein kinase binding, this GO :0044822 : poly(A) RNA binding, this GO :0051059 : NF-kappaB binding, this GO :0055105 : ubiquitin-protein transferase inhibitor activity, this GO :0097371 : MDM2/MDM4 family protein binding, cyclin-dependent kinase inhibitor 2A |
| Identity: |
1787 |
| Gene: |
CDKN2A |
More about : CDKN2A |
| Long gene name: |
cyclin dependent kinase inhibitor 2A |
| Synonyms gene: |
CDKN2 MLM |
| Synonyms gene name: |
cyclin-dependent kinase inhibitor 2A (melanoma, inhibits CDK4) , p16 |
| Synonyms: |
CDK4I p16 INK4a MTS1 CMM2 ARF p19 p14 INK4 p16INK4a p19Arf p14ARF |
| Locus: |
9p21, 3 |
| Discovery year: |
1994-05-19 |
| GenBank acession: |
L27211 |
| Entrez gene record: |
1029 |
| Pubmed identfication: |
8152487 7606716 |
| RefSeq identity: |
NM_000077 |
| Havana BLAST/BLAT: |
OTTHUMG00000019686 |
| Locus Specific Databases: |
LOVD - Leiden Open Variation Database LRG_11 |