| Antigen detectable with this ELISA kit: |
Cyclin Dependent Kinase 4 (CDK4) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity: |
114ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cyclin Dependent Kinase 4 (CDK4), The concentration of Cyclin Dependent Kinase 4 (CDK4) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin Dependent Kinase 4 (CDK4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Cyclin Dependent Kinase 4 (CDK4), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Cyclin Dependent Kinase 4 (CDK4) |
| Short name: |
Cyclin Dependent Kinase 4 (CDK4) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Cyclin Dependent phosphorylation catalyst 4 (cyclin-dependent kinase 4) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
CDK4 and IDBG-43642 and ENSG00000135446 and 1019, CMM3 and PSK-J3, Cdk4 and IDBG-194056 and ENSMUSG00000006728 and 102641469, nuclei, this GO :0000082 and G1/S transition of mitotic cell cycle and biological process this GO :0000278 and mitotic cell cycle and biological process this GO :0000307 and cyclin-dependent protein kinase holoenzyme complex and cellular component this GO :0000785 and chromatin and cellular component this GO :0002088 and lens development in camera-type eye and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004674 and protein serine/threonine kinase activity and molecular function this GO :0004693 and cyclin-dependent protein serine/threonine kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005667 and transcription factor complex and cellular component this GO :0005730 and nucleolus and cellular component this GO :0005829 and cytosol and cellular component this GO :0005923 and tight junction and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0007165 and signal transduction and biological process this GO :0007623 and circadian rhythm and biological process this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0009636 and response to toxic substance and biological process this GO :0010033 and response to organic substance and biological process this GO :0010288 and response to lead ion and biological process this GO :0010468 and regulation of gene expression and biological process this GO :0010971 and positive regulation of G2/M transition of mitotic cell cycle and biological process this GO :0016301 and kinase activity and molecular function this GO :0016772 and transferase activity, this GO :0004672 : protein kinase activity, this GO :0004672 : protein kinase activity and also this GO :0004674 : protein serine/threonine kinase activity and also this GO :0004693 : cyclin-dependent protein serine/threonine kinase activity and also this GO :0004713 : protein tyrosine kinase activity and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0016301 : kinase activity and also this GO :0016772 : transferase activity, this GO :0004674 : protein serine/threonine kinase activity, this GO :0004693 : cyclin-dependent protein serine/threonine kinase activity, this GO :0004713 : protein tyrosine kinase activity, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0016301 : kinase activity, this GO :0016772 : transferase activity, this GO :0030332 : cyclin binding, this GO :0032403 : protein complex binding, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups and also this GO :0030332 : cyclin binding and also this GO :0032403 : protein complex binding, transferring phosphorus-containing groups and molecular function this GO :0030332 and cyclin binding and molecular function this GO :0031100 and organ regeneration and biological process this GO :0031965 and nuclear membrane and cellular component this GO :0032403 and protein complex binding and molecular function this GO :0033574 and response to testosterone and biological process this GO :0042127 and regulation of cell proliferation and biological process this GO :0042493 and response to drug and biological process this GO :0043065 and positive regulation of apoptotic process and biological process this GO :0045727 and positive regulation of translation and biological process this GO :0045793 and positive regulation of cell size and biological process this GO :0048146 and positive regulation of fibroblast proliferation and biological process this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0051301 and cell division and biological process this GO :0051726 and regulation of cell cycle and biological process this GO :0055093 and response to hyperoxia and biological process this GO :0071157 and negative regulation of cell cycle arrest and biological process, 12567, cyclin-dependent kinase 4 |
| Identity: |
1773 |
| Gene: |
CDK4 |
More about : CDK4 |
| Long gene name: |
cyclin dependent kinase 4 |
| Synonyms: |
PSK-J3 |
| Locus: |
12q14, 1 |
| Discovery year: |
1993-07-28 |
| GenBank acession: |
M14505 |
| Entrez gene record: |
1019 |
| Pubmed identfication: |
8275715 |
| RefSeq identity: |
NM_000075 |
| Classification: |
Cyclin dependent kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000170382 |
| Locus Specific Databases: |
LRG_490 |