| Antigen detectable with this ELISA kit: |
Cyclin D1 (CCND1) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity: |
119ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell culture supernates and other biological fluids, cell lysates, plasma, tissue homogenates, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cyclin D1 (CCND1), The concentration of Cyclin D1 (CCND1) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin D1 (CCND1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Cyclin D1 (CCND1), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Cyclin D1 (CCND1) |
| Short name: |
Cyclin D1 (CCND1) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Cyclin D1 (cyclin D1) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
BCL1 and D11S287E and PRAD1 and U21B31, CCND1 and IDBG-61868 and ENSG00000110092 and 595, CCND1 and IDBG-645012 and ENSBTAG00000017514 and 524530, Ccnd1 and IDBG-212973 and ENSMUSG00000070348 and 12443, DNA-templated and biological process this GO :0006468 and protein phosphorylation and biological process this GO :0006974 and cellular response to DNA damage stimulus and biological process this GO :0007049 and cell cycle and biological process this GO :0007219 and Notch signaling pathway and biological process this GO :0007595 and lactation and biological process this GO :0008134 and transcription factor binding and molecular function this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0010033 and response to organic substance and biological process this GO :0010039 and response to iron ion and biological process this GO :0010165 and response to X-ray and biological process this GO :0010243 and response to organonitrogen compound and biological process this GO :0010971 and positive regulation of G2/M transition of mitotic cell cycle and biological process this GO :0014070 and response to organic cyclic compound and biological process this GO :0016020 and membrane and cellular component this GO :0016055 and Wnt signaling pathway and biological process this GO :0016301 and kinase activity and molecular function this GO :0016538 and cyclin-dependent protein serine/threonine kinase regulator activity and molecular function this GO :0017053 and transcriptional repressor complex and cellular component this GO :0019899 and enzyme binding and molecular function this GO :0019901 and protein kinase binding and molecular function this GO :0030178 and negative regulation of Wnt signaling pathway and biological process this GO :0030857 and negative regulation of epithelial cell differentiation and biological process this GO :0030968 and endoplasmic reticulum unfolded protein response and biological process this GO :0031100 and organ regeneration and biological process this GO :0031571 and mitotic G1 DNA damage checkpoint and biological process this GO :0032026 and response to magnesium ion and biological process this GO :0032403 and protein complex binding and molecular function this GO :0033197 and response to vitamin E and biological process this GO :0033327 and Leydig cell differentiation and biological process this GO :0033598 and mammary gland epithelial cell proliferation and biological process this GO :0033601 and positive regulation of mammary gland epithelial cell proliferation and biological process this GO :0042493 and response to drug and biological process this GO :0042826 and histone deacetylase binding and molecular function this GO :0043627 and response to estrogen and biological process this GO :0045444 and fat cell differentiation and biological process this GO :0045471 and response to ethanol and biological process this GO :0045737 and positive regulation of cyclin-dependent protein serine/threonine kinase activity and biological process this GO :0048545 and response to steroid hormone and biological process this GO :0051301 and cell division and biological process this GO :0051384 and response to glucocorticoid and biological process this GO :0051412 and response to corticosterone and biological process this GO :0051592 and response to calcium ion and biological process this GO :0051726 and regulation of cell cycle and biological process this GO :0060070 and canonical Wnt signaling pathway and biological process this GO :0060749 and mammary gland alveolus development and biological process this GO :0070064 and proline-rich region binding and molecular function this GO :0070141 and response to UV-A and biological process this GO :0071157 and negative regulation of cell cycle arrest and biological process this GO :0071310 and cellular response to organic substance and biological process this GO :2000045 and regulation of G1/S transition of mitotic cell cycle and biological process, nuclei, proline-rich region binding, this GO :0000079 and regulation of cyclin-dependent protein serine/threonine kinase activity and biological process this GO :0000082 and G1/S transition of mitotic cell cycle and biological process this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0000278 and mitotic cell cycle and biological process this GO :0000307 and cyclin-dependent protein kinase holoenzyme complex and cellular component this GO :0000320 and re-entry into mitotic cell cycle and biological process this GO :0001889 and liver development and biological process this GO :0001934 and positive regulation of protein phosphorylation and biological process this GO :0003714 and transcription corepressor activity and molecular function this GO :0004672 and protein kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005622 and intracellular and cellular component this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0005923 and tight junction and cellular component this GO :0006351 and transcription, this GO :0003714 : transcription corepressor activity, this GO :0003714 : transcription corepressor activity and also this GO :0004672 : protein kinase activity and also this GO :0005515 : protein binding and also this GO :0008134 : transcription factor binding and also this GO :0016301 : kinase activity and also this GO :0016538 : cyclin-dependent protein serine/threonine kinase regulator activity and also this GO :0019899 : enzyme binding and also this GO :0019901 : protein kinase binding and also this GO :0032403 : protein complex binding and also this GO :0042826 : histone deacetylase binding and also this GO :0070064 : proline-rich region binding, this GO :0004672 : protein kinase activity, this GO :0005515 : protein binding, this GO :0008134 : transcription factor binding, this GO :0016301 : kinase activity, this GO :0016538 : cyclin-dependent protein serine/threonine kinase regulator activity, this GO :0019899 : enzyme binding, this GO :0019901 : protein kinase binding, this GO :0032403 : protein complex binding, this GO :0042826 : histone deacetylase binding, this GO :0070064 : proline-rich region binding, cyclin D1 |
| Identity: |
1582 |
| Gene: |
CCND1 |
More about : CCND1 |
| Long gene name: |
cyclin D1 |
| Synonyms gene: |
BCL1 D11S287E PRAD1 |
| Synonyms gene name: |
cyclin D1 (PRAD1: parathyroid adenomatosis 1) |
| Synonyms: |
U21B31 |
| Synonyms name: |
parathyroid adenomatosis 1 B-cell CLL/lymphoma 1 G1/S-specific cyclin D1 |
| Locus: |
11q13, 3 |
| Discovery year: |
1991-06-06 |
| GenBank acession: |
Z23022 |
| Entrez gene record: |
595 |
| Pubmed identfication: |
1826542 1833066 |
| RefSeq identity: |
NM_053056 |
| Classification: |
Cyclins |
| Havana BLAST/BLAT: |
OTTHUMG00000167877 |
| Locus Specific Databases: |
LRG_990 |