| Antigen detectable with this ELISA kit: |
Crk Like Protein (CRKL) |
| Species reactivity: |
Mus musculus (Mouse) |
| Range of detection: |
1250pg/mL, 156, 2500pg/mL, 312, 625pg/mL, 78, 125-5000pg/mL The standard curve concentrations used for the ELISA&rsquo, 125pg/mL, 25pg/mL, 5pg/mL, 78, s were 5000pg/mL |
| Sensitivity: |
The minimum detectable dose of this kit is typically less than 33pg/mL |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Crk Like Protein (CRKL), The concentration of Crk Like Protein (CRKL) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Crk Like Protein (CRKL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Crk Like Protein (CRKL), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Crk Like Protein (CRKL) |
| Short name: |
Crk Like Protein (CRKL) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Crk Like Protein (v-crk sarcoma virus CT10 oncogene homolog (avian)-like) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
CRKL and IDBG-1833 and ENSG00000099942 and 1399, CRKL and IDBG-637664 and ENSBTAG00000045548 and, Crkl and IDBG-138913 and ENSMUSG00000006134 and 12929, Cytoplasm, poly(A) RNA binding, this GO :0000186 and activation of MAPKK activity and biological process this GO :0001568 and blood vessel development and biological process this GO :0004871 and signal transducer activity and molecular function this GO :0005070 and SH3/SH2 adaptor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005768 and endosome and cellular component this GO :0005829 and cytosol and cellular component this GO :0007254 and JNK cascade and biological process this GO :0007265 and Ras protein signal transduction and biological process this GO :0007389 and pattern specification process and biological process this GO :0007507 and heart development and biological process this GO :0009887 and organ morphogenesis and biological process this GO :0009952 and anterior/posterior pattern specification and biological process this GO :0009967 and positive regulation of signal transduction and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0044822 and poly(A) RNA binding and molecular function this GO :0045087 and innate immune response and biological process this GO :0048011 and neurotrophin TRK receptor signaling pathway and biological process this GO :0048538 and thymus development and biological process this GO :0060017 and parathyroid gland development and biological process this GO :0070062 and extracellular vesicular exosome and cellular component, this GO :0004871 : signal transducer activity, this GO :0004871 : signal transducer activity and also this GO :0005070 : SH3/SH2 adaptor activity and also this GO :0005515 : protein binding and also this GO :0044822 : poly(A) RNA binding, this GO :0005070 : SH3/SH2 adaptor activity, this GO :0005515 : protein binding, this GO :0044822 : poly(A) RNA binding, v-crk sarcoma virus CT10 oncogene homolog (avian)-like |
| Identity: |
2363 |
| Gene: |
CRKL |
More about : CRKL |
| Long gene name: |
CRK like proto-oncogene, adaptor protein |
| Synonyms gene name: |
v-crk avian sarcoma virus CT10 oncogene homolog-like |
| Locus: |
22q11, 21 |
| Discovery year: |
1994-09-14 |
| Entrez gene record: |
1399 |
| Pubmed identfication: |
8361759 8798523 |
| RefSeq identity: |
NM_005207 |
| Classification: |
SH2 domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000150807 |