| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
Biotin |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
RanBP3 |
| Specificity: |
This antibody reacts specifically with RanBP3 Biotin |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human RanBP3 |
| Gene ID: |
8498 |
| Swiss Prot: |
Q9H6Z4 |
| Applications: |
IHC-P, WB |
| Applications with corresponding dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Binds to CHC1/RCC1 and increases the guanine nucleotide exchange activity of CHC1/RCC1, Bound to XPO1/CRM1, In the absence of Ran-bound GTP prevents binding of XPO1/CRM1 to the nuclear pore complex, Negative regulator of TGF-beta signaling through interaction with the R-SMAD proteins, Recruits XPO1/CRM1 to CHC1/RCC1 in a Ran-dependent manner, SMAD2 and SMAD3, and mediating their nuclear export, perhaps as export complex scaffolding protein, stabilizes the XPO1/CRM1-cargo interaction, Acts as a cofactor for XPO1/CRM1-mediated nuclear export |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Also known as: |
RanBP3 Polyclonal Antibody Biotin |
| Other name: |
Anti-RanBP3 Polyclonal Biotin |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, Biotin conjugates can be detected by horseradish peroxidase |
| Conjugation: |
Biotinylated |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
RanBP3 |
| Short name: |
Anti-RanBP3 (Polyclonal) |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
Biotin |
| Alternative name: |
biotinilated, antibody to-RanBP3 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
9850 |
| Gene: |
RANBP3 |
More about : RANBP3 |
| Long gene name: |
RAN binding protein 3 |
| Locus: |
19p13, 3 |
| Discovery year: |
1998-11-30 |
| GenBank acession: |
Y08698 |
| Entrez gene record: |
8498 |
| Pubmed identfication: |
9637251 |
| RefSeq identity: |
NM_007322 |
| Havana BLAST/BLAT: |
OTTHUMG00000180636 |