| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
LCAT |
| Specificity: |
This antibody reacts specifically with LCAT |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Secreted |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human LCAT |
| Gene ID: |
3931 |
| Swiss Prot: |
P04180 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Also produced in the brain by primary astrocytes, Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines, Required for remodeling high-density lipoprotein particles into their spherical forms, Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs), The cholesterol ester is then transported back to the liver, Together with APOE and the cholesterol transporter ABCA1, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels, nascent lipoproteins, plays a key role in the maturation of glial-derived, Central enzyme in the extracellular metabolism of plasma lipoproteins |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
LCAT Polyclonal Antibody |
| Other name: |
Anti-LCAT Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
LCAT |
| Short name: |
Anti-LCAT (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-LCAT (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
6522 |
| Gene: |
LCAT |
More about : LCAT |
| Long gene name: |
lecithin-cholesterol acyltransferase |
| Synonyms name: |
phosphatidylcholine--sterol O-acyltransferase |
| Locus: |
16q22, 1 |
| Discovery year: |
2001-06-22 |
| Entrez gene record: |
3931 |
| Havana BLAST/BLAT: |
OTTHUMG00000137551 |