| Antigen detectable with this ELISA kit: |
Complement Factor H (CFH) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
23, 187, 375ng/mL, 46, 750ng/mL, 23, 93, 438-1500ng/mL The standard curve concentrations used for the ELISA&rsquo, 438ng/mL, 5ng/mL, 75ng/mL, 875ng/mL, s were 1500ng/mL |
| Sensitivity: |
75ng/mL, The minimum detectable dose of this kit is typically less than 8 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Factor H (CFH), The concentration of Complement Factor H (CFH) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Factor H (CFH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Complement Factor H (CFH), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Complement Factor H (CFH) |
| Short name: |
Complement Factor H (CFH) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Complement Factor H (complement factor H) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
AHUS1 and AMBP1 and ARMD4 and ARMS1 and CFHL3 and FH and FHL1 and HF and HF1 and HF2 and HUS, CFH and IDBG-105504 and ENSG00000000971 and 3075, Extracellular, alternative pathway and biological process this GO :0008201 and heparin binding and molecular function this GO :0030449 and regulation of complement activation and biological process this GO :0043395 and heparan sulfate proteoglycan binding and molecular function this GO :0045087 and innate immune response and biological process this GO :0072562 and blood microparticle and cellular component, heparan sulfate proteoglycan binding, this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0006956 and complement activation and biological process this GO :0006957 and complement activation, this GO :0005515 : protein binding, this GO :0005515 : protein binding and also this GO :0008201 : heparin binding and also this GO :0043395 : heparan sulfate proteoglycan binding, this GO :0008201 : heparin binding, this GO :0043395 : heparan sulfate proteoglycan binding, complement factor H |
| Identity: |
4883 |
| Gene: |
CFH |
More about : CFH |
| Long gene name: |
complement factor H |
| Synonyms gene: |
HF HF1 HF2 |
| Synonyms gene name: |
H factor 1 (complement) |
| Synonyms: |
HUS FHL1 ARMS1 ARMD4 |
| Synonyms name: |
beta-1H H factor 2 (complement) age-related maculopathy susceptibility 1 |
| Locus: |
1q31, 3 |
| Discovery year: |
1986-01-01 |
| GenBank acession: |
Y00716 |
| Entrez gene record: |
3075 |
| Pubmed identfication: |
2889480 2963625 |
| RefSeq identity: |
NM_000186 |
| Classification: |
Sushi domain containing Complement system |
| Havana BLAST/BLAT: |
OTTHUMG00000035607 |
| Locus Specific Databases: |
CFHbase: Mutation registry for Factor H deficiency (previously known as HF1base) LRG_47 |