| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Bcl-2 |
| Specificity: |
This antibody reacts specifically with Bcl-2 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Membrane bound |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Bcl-2 |
| Gene ID: |
596 |
| Swiss Prot: |
P10415 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Appears to function in a feedback loop system with caspases, Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1), Regulates cell death by controlling the mitochondrial membrane permeability, Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
Bcl-2 PPP1R50 |
| Also known as: |
Bcl-2 Polyclonal Antibody |
| Other name: |
Anti-Bcl-2 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Bcl-2 |
| Short name: |
Anti-Bcl-2 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Bcl-2 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
990 |
| Gene: |
BCL2 |
More about : BCL2 |
| Long gene name: |
BCL2, apoptosis regulator |
| Synonyms gene name: |
B-cell CLL/lymphoma 2 |
| Synonyms name: |
protein phosphatase 1, regulatory subunit 50 |
| Locus: |
18q21, 33 |
| Discovery year: |
2001-06-22 |
| GenBank acession: |
M14745 |
| Entrez gene record: |
596 |
| RefSeq identity: |
NM_000633 NM_000657 |
| Classification: |
Protein phosphatase 1 regulatory subunits BCL2 family |
| Havana BLAST/BLAT: |
OTTHUMG00000132791 |
| Locus Specific Databases: |
LRG_1113 |