| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
HHAT |
| Specificity: |
This antibody reacts specifically with HHAT |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human HHAT |
| Gene ID: |
55733 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Chromosome 1 is the largest human chromosome, Expressed ubiquitously, Gaucher disease, HHAT functions to catalyze the N-terminal palmitoylation of SSH (slingshot homolog), HHAT is expressed in cancer cell lines, Parkinsons Disease, Several disorders, The gene encoding HHAT maps to human chromosome 1 and is expressed as four alternatively spliced isoforms, an event that is required for SHH signaling pathways, are caused by defects in genes that localize to chromosome 1, including Stickler syndrome, malignant melanoma and Usher syndrome, spanning about 260 million base pairs and making up 8% of the human genome, suggesting a role for HHAT in tumorigenesis, HHAT is a 493 amino acid multi-pass membrane protein that localizes to the endoplasmic reticulum and belongs to the membrane-bound acyltransferase family |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
FLJ10724 MART-2 MART2 Skn ski rasp sit GUP2 |
| Also known as: |
HHAT Polyclonal Antibody |
| Other name: |
Anti-HHAT Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
HHAT |
| Short name: |
Anti-HHAT (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-HHAT (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
18270 |
| Gene: |
HHAT |
More about : HHAT |
| Long gene name: |
hedgehog acyltransferase |
| Locus: |
1q32, 2 |
| Discovery year: |
2004-09-15 |
| GenBank acession: |
AK001586 |
| Entrez gene record: |
55733 |
| Pubmed identfication: |
11160356 |
| RefSeq identity: |
NM_018194 |
| Classification: |
Membrane bound O-acyltransferases |
| Havana BLAST/BLAT: |
OTTHUMG00000036447 |