| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
SUMO 1 |
| Specificity: |
There is a chance that this antibody will cross-react with SUMO13SUMO 1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human SUMO 1 |
| Gene ID: |
7341 |
| Swiss Prot: |
P63165 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, DNA replication and repair, Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2, May also regulate a network of genes involved in palate development, Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins, RANBP2 or CBX4, This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, and can be promoted by E3 ligases such as PIAS1-4, mitosis and signal transduction, Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
AOS1 FLJ3091 Sua1 |
| Also known as: |
SUMO 1 Polyclonal Antibody |
| Other name: |
Anti-SUMO 1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
SUMO 1 |
| Short name: |
Anti-SUMO 1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-SUMO 1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
30660 |
| Gene: |
SAE1 |
More about : SAE1 |
| Long gene name: |
SUMO1 activating enzyme subunit 1 |
| Synonyms name: |
activator Of sumo 1 |
| Locus: |
19q13, 32 |
| Discovery year: |
2006-10-31 |
| GenBank acession: |
BC018271 |
| Entrez gene record: |
10055 |
| Pubmed identfication: |
10187858 9920803 10217437 |
| RefSeq identity: |
NM_005500 |
| Classification: |
Ubiquitin like modifier activating enzymes |
| Havana BLAST/BLAT: |
OTTHUMG00000183464 |