| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
E2AK2/PKR |
| Specificity: |
This antibody reacts specifically with EIF2AK2/PKR |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human PKR |
| Gene ID: |
5610 |
| Swiss Prot: |
P19525 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
ATF3) involved in the expression of genes encoding proinflammatory cytokines and IFNs, Activates the NF-kappa-B pathway via interaction with IKBKB and TRAF family of proteins and activates the p38 MAP kinase pathway via interaction with MAP2K6, Also phosphorylates other substrates including p53/TP53, Can act as both a positive and negative regulator of the insulin signaling pathway (ISP), DHX9, Either as an adapter protein and/or via its kinase activity, Exerts its antiviral activity on a wide range of DNA and RNA viruses including hepatitis C virus (HCV), ILF3, IRF1, IRS1 and the HHV-1 viral protein US11, In addition to serine/threonine-protein kinase activity, Inhibits viral replication via phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (EIF2S1), NF-kappa-B and insulin signaling pathways) and transcription factors (JUN, Negatively regulates ISP by inducing the inhibitory phosphorylation of insulin receptor substrate 1 (IRS1) at 'Ser-312' and positively regulates ISP via phosphorylation of PPP2R5A which activates FOXO1, PPP2R5A, STAT1, STAT3, also has tyrosine-protein kinase activity and phosphorylates CDK1 at 'Tyr-4' upon DNA damage, apoptosis, can regulate various signaling pathways (p38 MAP kinase, cell proliferation and differentiation, facilitating its ubiquitination and proteosomal degradation, hepatitis B virus (HBV), measles virus (MV) and herpes simplex virus 1 (HHV-1), this phosphorylation impairs the recycling of EIF2S1 between successive rounds of initiation leading to inhibition of translation which eventually results in shutdown of cellular and viral protein synthesis, which in turn up-regulates the expression of insulin receptor substrate 2 (IRS2), IFN-induced dsRNA-dependent serine/threonine-protein kinase which plays a key role in the innate immune response to viral infection and is also involved in the regulation of signal transduction |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
PKR EIF2AK1 PPP1R83 |
| Also known as: |
EIF2AK2/PKR Polyclonal Antibody |
| Other name: |
Anti-EIF2AK2/PKR Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
EIF2AK2/PKR |
| Short name: |
Anti-EIF2AK2/PKR (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-eukaryotic translation initiation factor 2-a phosphorylation catalyst 2/PKR (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
DNA-templated and biological process this GO :0006412 and translation and biological process this GO :0006468 and protein phosphorylation and biological process this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0008601 and protein phosphatase type 2A regulator activity and molecular function this GO :0009615 and response to virus and biological process this GO :0016020 and membrane and cellular component this GO :0016772 and transferase activity, EIF2AK1 and PKR and PPP1R83 and PRKR, EIF2AK2 and IDBG-44867 and ENSG00000055332 and 5610, EIF2AK2 and IDBG-632472 and ENSBTAG00000008703 and 347700, Eif2ak2 and IDBG-199014 and ENSMUSG00000024079 and 19106, nuclei, this GO :0000186 and activation of MAPKK activity and biological process this GO :0001819 and positive regulation of cytokine production and biological process this GO :0003725 and double-stranded RNA binding and molecular function this GO :0004672 and protein kinase activity and molecular function this GO :0004674 and protein serine/threonine kinase activity and molecular function this GO :0004694 and eukaryotic translation initiation factor 2alpha kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0004715 and non-membrane spanning protein tyrosine kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0006351 and transcription, this GO :0003725 : double-stranded RNA binding, this GO :0003725 : double-stranded RNA binding and also this GO :0004672 : protein kinase activity and also this GO :0004674 : protein serine/threonine kinase activity and also this GO :0004694 : eukaryotic translation initiation factor 2alpha kinase activity and also this GO :0004713 : protein tyrosine kinase activity and also this GO :0004715 : non-membrane spanning protein tyrosine kinase activity and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0008601 : protein phosphatase type 2A regulator activity and also this GO :0016772 : transferase activity, this GO :0004672 : protein kinase activity, this GO :0004674 : protein serine/threonine kinase activity, this GO :0004694 : eukaryotic translation initiation factor 2alpha kinase activity, this GO :0004713 : protein tyrosine kinase activity, this GO :0004715 : non-membrane spanning protein tyrosine kinase activity, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0008601 : protein phosphatase type 2A regulator activity, this GO :0016772 : transferase activity, this GO :0044822 : poly(A) RNA binding, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups and also this GO :0044822 : poly(A) RNA binding, transferring phosphorus-containing groups and molecular function this GO :0017148 and negative regulation of translation and biological process this GO :0018108 and peptidyl-tyrosine phosphorylation and biological process this GO :0019048 and modulation by virus of host morphology or physiology and biological process this GO :0019054 and modulation by virus of host process and biological process this GO :0019058 and viral life cycle and biological process this GO :0030683 and evasion or tolerance by virus of host immune response and biological process this GO :0030968 and endoplasmic reticulum unfolded protein response and biological process this GO :0032722 and positive regulation of chemokine production and biological process this GO :0032874 and positive regulation of stress-activated MAPK cascade and biological process this GO :0033689 and negative regulation of osteoblast proliferation and biological process this GO :0035455 and response to interferon-alpha and biological process this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0044822 and poly(A) RNA binding and molecular function this GO :0045071 and negative regulation of viral genome replication and biological process this GO :0045087 and innate immune response and biological process this GO :0046777 and protein autophosphorylation and biological process this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0051092 and positive regulation of NF-kappaB transcription factor activity and biological process this GO :0051607 and defense response to virus and biological process this GO :1900225 and regulation of NLRP3 inflammasome complex assembly and biological process this GO :1901224 and positive regulation of NIK/NF-kappaB signaling and biological process this GO :1901532 and regulation of hematopoietic progenitor cell differentiation and biological process this GO :1902033 and regulation of hematopoietic stem cell proliferation and biological process this GO :1902036 and regulation of hematopoietic stem cell differentiation and biological process, eukaryotic translation initiation factor 2-alpha kinase 2 |
| Identity: |
9437 |
| Gene: |
EIF2AK2 |
More about : EIF2AK2 |
| Long gene name: |
eukaryotic translation initiation factor 2 alpha kinase 2 |
| Synonyms gene: |
PRKR |
| Synonyms gene name: |
interferon-inducible double stranded RNA dependent , protein kinase |
| Synonyms name: |
protein phosphatase 1, regulatory subunit 83 |
| Locus: |
2p22, 2 |
| Discovery year: |
1992-12-04 |
| GenBank acession: |
BC057805 |
| Entrez gene record: |
5610 |
| Pubmed identfication: |
1351683 |
| RefSeq identity: |
NM_002759 |
| Classification: |
Protein phosphatase 1 regulatory subunits |
| Havana BLAST/BLAT: |
OTTHUMG00000100962 |