| Antigen detectable with this ELISA kit: |
Coagulation Factor VIII (F8) |
| Species reactivity: |
Canine (Dog), Canis familiaris |
| Range of detection: |
100ng/mL, 12, 200ng/mL, 25ng/mL, 50ng/mL, 6, 25-400ng/mL The standard curve concentrations used for the ELISA&rsquo, 25ng/mL, 5ng/mL, 6, s were 400ng/mL |
| Sensitivity: |
39ng/mL, The minimum detectable dose of this kit is typically less than 2 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Plasma |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coagulation Factor VIII (F8), The concentration of Coagulation Factor VIII (F8) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor VIII (F8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Coagulation Factor VIII (F8), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Coagulation Factor VIII (F8) |
| Short name: |
Coagulation Factor VIII (F8) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
procoagulant component) Enzyme-linked immunosorbent assay reagent, Coagulation Factor VIII (coagulation factor VIII |
| Alternative technique: |
elisas |
| Alternative to gene target: |
AHF and DXS1253E and F8B and F8C and FVIII and HEMA, Extracellular, F8 and IDBG-157124 and ENSMUSG00000031196 and 14069, F8 and IDBG-632125 and ENSBTAG00000010726 and 100271720, F8 and IDBG-92134 and ENSG00000185010 and 2157, intrinsic pathway and biological process this GO :0016491 and oxidoreductase activity and molecular function this GO :0030168 and platelet activation and biological process this GO :0031093 and platelet alpha granule lumen and cellular component this GO :0055114 and oxidation-reduction process and biological process, oxidoreductase activity, procoagulant component, this GO :0002576 and platelet degranulation and biological process this GO :0004252 and serine-type endopeptidase activity and molecular function this GO :0005507 and copper ion binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006508 and proteolysis and biological process this GO :0006953 and acute-phase response and biological process this GO :0007155 and cell adhesion and biological process this GO :0007596 and blood coagulation and biological process this GO :0007597 and blood coagulation, this GO :0004252 : serine-type endopeptidase activity, this GO :0004252 : serine-type endopeptidase activity and also this GO :0005507 : copper ion binding and also this GO :0005515 : protein binding and also this GO :0016491 : oxidoreductase activity, this GO :0005507 : copper ion binding, this GO :0005515 : protein binding, this GO :0016491 : oxidoreductase activity, coagulation factor VIII |
| Identity: |
2294 |
| Gene: |
COX8A |
More about : COX8A |
| Long gene name: |
cytochrome c oxidase subunit 8A |
| Synonyms gene: |
COX8 |
| Synonyms gene name: |
cytochrome c oxidase subunit VIII cytochrome c oxidase subunit VIIIA (ubiquitous) |
| Synonyms: |
COX8-2 COX8L VIII-L COX VIII |
| Locus: |
11q13, 1 |
| Discovery year: |
1989-06-30 |
| GenBank acession: |
J04823 |
| Entrez gene record: |
1351 |
| Pubmed identfication: |
2543673 2847943 |
| RefSeq identity: |
NM_004074 |
| Classification: |
Mitochondrial complex IV: cytochrome c oxidase subunits |
| Havana BLAST/BLAT: |
OTTHUMG00000167785 |