| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
SREBP-1/2 |
| Specificity: |
This antibody reacts specifically with SREBP-1/2 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Intracellular |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human SREBP-1 |
| Gene ID: |
6720, 6721 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Belongs to the SREBP family, Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'), COPII-coated vesicle membrane, Cytoplasmic vesicle, Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein, Golgi apparatus membrane, Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3'), Interacts with LMNA, Isoform SREBP-1A is much more active than isoform SREBP-1C in stimulating transcription from SRE-1-containing promoters, Multi-pass membrane protein, Multi-pass membrane protein, Multi-pass membrane protein, Note=Moves from the endoplasmic reticulum to the Golgi in the absence of sterols, Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway, [SUBCELLULAR LOCATION] Endoplasmic reticulum membrane, [SUBCELLULAR LOCATION] Processed sterol regulatory element-binding protein 1: Nucleus, [SUBUNIT] Forms a tight complex with SCAP in the ER membrane, Transcriptional activator required for lipid homeostasis |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
ADD 1, BHLHD1, D630008H06, SREBF 1, SREBF1, SREBP 1, SREBP 1c, SREBP1, Sterol Regulatory Element Binding Transcription Factor 1 / Protein 1, Sterol regulatory element binding protein 1, Sterol regulatory element binding transcription factor 1, Sterol regulatory element binding protein 1 |
| Also known as: |
SREBP-1/2 Polyclonal Antibody |
| Other name: |
Anti-SREBP-1/2 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
SREBP-1/2 |
| Short name: |
Anti-SREBP-1/2 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-SREBP-1/2 (polyclonal) |
| Alternative technique: |
polyclonals |