| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
CDC34/UBC3 |
| Specificity: |
This antibody reacts specifically with CDC34/UBC3 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Cdc34 |
| Gene ID: |
997 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
A key negative regulator of mitosis, Mutants in the gene Cdc 34 of the yeast Saccharomyces cerevisiae are defective in the transition from G1 to the S phase of the cell cycle, The cell cycle function of Cdc 34 is thus likely to be mediated by the ubiquitin conjugating activity of its product, The human Cdc 34 gene is located on the far telomeric region of 19p13, Therefore, This gene was cloned and shown to encode a 295 residue protein that has substantial sequence similarity to yeast Rad6 protein required for a variety of cellular functions including DNA repair and was recently shown to encode a ubiquitin conjugating enzyme, This proteolysis event is required for a timely entrance into mitosis and is inhibited when DNA replication is blocked, When produced in Escherichia coli, demonstrating that the Cdc 34 protein is another distinct member of the family of ubiquitin conjugating enzymes, is degraded in a Cdc 34 dependent fashion in Xenopus egg extracts, the Cdc 34 gene product catalyzes the covalent attachment of ubiquitin to histones H2A and H2B in vitro, the DNA replication checkpoint can prevent mitosis by suppressing the proteolysis of Wee 1 during S phase, the protein kinase Wee 1, The Cdc 34 gene represents a mammalian homolog of the group of yeast Cdc 34 genes required for the late G1 - S phase transition |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
E2-CDC34 UBE2R1 UBC3 |
| Also known as: |
CDC34/UBC3 Polyclonal Antibody |
| Other name: |
Anti-CDC34/UBC3 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
CDC34/UBC3 |
| Short name: |
Anti-CDC34/UBC3 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-CDC34/UBC3 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
1734 |
| Gene: |
CDC34 |
More about : CDC34 |
| Long gene name: |
cell division cycle 34 |
| Synonyms gene name: |
cell division cycle 34 cell division cycle 34 homolog (S, cerevisiae) |
| Synonyms name: |
ubiquitin conjugating enzyme E2 R1 |
| Locus: |
19p13, 3 |
| Discovery year: |
1994-05-12 |
| GenBank acession: |
L22005 |
| Entrez gene record: |
997 |
| Pubmed identfication: |
8248134 16210246 |
| RefSeq identity: |
NM_004359 |
| Classification: |
Ubiquitin conjugating enzymes E2 |
| Havana BLAST/BLAT: |
OTTHUMG00000180558 |