| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
ATG4B |
| Specificity: |
This antibody reacts specifically with ATG4B |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human ATG4B |
| Gene ID: |
23192 |
| Swiss Prot: |
Q9Y4P1 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, GABARAPL1, GABARAPL2 and GABARAP, Has also an activity of delipidating enzyme for the PE-conjugated forms, to reveal a C-terminal glycine, which is necessary for autophagy, Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
Apg4B KIAA0943 DKFZp586D1822 AUTL1 |
| Also known as: |
ATG4B Polyclonal Antibody |
| Other name: |
Anti-ATG4B Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
ATG4B |
| Short name: |
Anti-ATG4B (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-ATG4B (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
20790 |
| Gene: |
ATG4B |
More about : ATG4B |
| Long gene name: |
autophagy related 4B cysteine peptidase |
| Synonyms gene: |
APG4B |
| Synonyms gene name: |
APG4 autophagy 4 homolog B (S, cerevisiae) ATG4 autophagy related 4 homolog B (S, cerevisiae) autophagy related 4B, cysteine peptidase |
| Locus: |
2q37, 3 |
| Discovery year: |
2004-03-23 |
| GenBank acession: |
AB023160 |
| Entrez gene record: |
23192 |
| Pubmed identfication: |
12446702 |
| RefSeq identity: |
NM_013325 |
| Classification: |
Autophagy related |
| Havana BLAST/BLAT: |
OTTHUMG00000151514 |