| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Smoothelin |
| Specificity: |
This antibody reacts specifically with Smoothelin |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Smoothelin |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
As a marker for the highly differentiated contractile smooth muscle cell, At least two isoforms of smoothelin are produced by alternative splicing, In the vascular system, Smoothelin associates with actin stress fibers but does not interact with desmin, The gene encoding human smoothelin maps to chromosome 22q12, The short isoform is expressed in visceral muscle tissue, The short isoform lacks amino acids 1â, but not in brain, including intestine and stomach, smoothelin expression is limited to large veins and arteries capable of pulsatile contraction, smoothelin expression is useful for studying vascular malformation and injury, while the long isoform is expressed in all vascularized organs, &euro, &ldquo, 2, 544 at the amino-terminus of the long isoform, The cytoskeletal protein smoothelin is highly conserved among vertebrates and is expressed exclusively by contractile smooth muscle cells where it localizes to the filament network |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
Smoothelin Polyclonal Antibody |
| Other name: |
Anti-Smoothelin Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Smoothelin |
| Short name: |
Anti-Smoothelin (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Smoothelin (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
11126 |
| Gene: |
SMTN |
More about : SMTN |
| Long gene name: |
smoothelin |
| Locus: |
22q12, 2 |
| Discovery year: |
1997-02-03 |
| GenBank acession: |
AY061972 |
| Entrez gene record: |
6525 |
| Pubmed identfication: |
9244445 8707825 |
| RefSeq identity: |
NM_134270 |
| Havana BLAST/BLAT: |
OTTHUMG00000151203 |