| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
SIT |
| Specificity: |
This antibody reacts specifically with SIT |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human SIT |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
In addition, It contains five potential tyrosine phosphorylation sites, Lck and ZAP-70, One such protein, SIT (SHP2 interacting transmembrane adaptor protein) is a disulfide-linked homodimeric glycoprotein that is expressed in lymphocytes, SIT interacts with SHP2 and also with the adaptor protein Grb2, SIT is reduced to half its molecular mass via endoglycosidase treatment, it is a substrate for the Src protein kinases Fyn, suggesting a role in TCR-mediated recruitment of SH2 domain-containing intracellular signaling molecules to the plasma membrane, T lymphocytes express several low molecular mass transmembrane adaptor proteins that recruit SH2 domain-containing intracellular molecules to the cell membrane via tyrosine-based signaling pathways |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
SIT |
| Also known as: |
SIT Polyclonal Antibody |
| Other name: |
Anti-SIT Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
SIT |
| Short name: |
Anti-SIT (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-SIT (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
17710 |
| Gene: |
SIT1 |
More about : SIT1 |
| Long gene name: |
signaling threshold regulating transmembrane adaptor 1 |
| Synonyms gene name: |
suppression inducing transmembrane adaptor 1 |
| Synonyms name: |
SHP2 interacting transmembrane adaptor |
| Locus: |
9p13, 3 |
| Discovery year: |
2005-04-25 |
| Entrez gene record: |
27240 |
| Pubmed identfication: |
11491537 10209036 |
| RefSeq identity: |
NM_014450 |
| Havana BLAST/BLAT: |
OTTHUMG00000019867 |