| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
GADD153/DDIT3 |
| Specificity: |
This antibody reacts specifically with GADD153/DDIT3 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human DDIT3 |
| Gene ID: |
1649 |
| Swiss Prot: |
P35638 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
ATF4-dependent transcriptional activation of asparagine synthetase (ASNS), Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, BBC3/PUMA, BCL2L11/BIM and ERO1L, CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG), IL23, IL6, IL8, Inhibits the canonical Wnt signaling pathway by binding to TCF7L2/TCF4, Negatively regulates, PPP1R15A/GADD34, Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes, Plays a regulatory role in the inflammatory response through the induction of caspase-11 (CASP4/CASP11) which induces the activation of caspase-1 (CASP1) and both these caspases increase the activation of pro-IL1B to mature IL1B which is involved in the inflammatory response, Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress, Positively regulates the transcription of TRIB3, TNFRSF1B/DR5, and inhibits the expression of C/EBP regulated genes, expression of BCL2 and MYOD1, impairing its DNA-binding properties and repressing its transcriptional activity, impairs their association with C/EBP binding sites in the promoter regions, Multifunctional transcription factor in ER stress response |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
CHOP10 GADD153 CHOP |
| Also known as: |
GADD153/DDIT3 Polyclonal Antibody |
| Other name: |
Anti-GADD153/DDIT3 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
GADD153/DDIT3 |
| Short name: |
Anti-GADD153/DDIT3 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-GADD153/Desoxyribonucleic acid-damage-inducible transcript 3 (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
CEBPZ and CHOP and CHOP-10 and CHOP10 and GADD153, DDIT3 and IDBG-42805 and ENSG00000175197 and 1649, DDIT3 and IDBG-635159 and ENSBTAG00000031544 and 777788, DNA-templated and biological process this GO :0006915 and apoptotic process and biological process this GO :0006974 and cellular response to DNA damage stimulus and biological process this GO :0006983 and ER overload response and biological process this GO :0006986 and response to unfolded protein and biological process this GO :0006987 and activation of signaling protein activity involved in unfolded protein response and biological process this GO :0007050 and cell cycle arrest and biological process this GO :0008134 and transcription factor binding and molecular function this GO :0016055 and Wnt signaling pathway and biological process this GO :0030968 and endoplasmic reticulum unfolded protein response and biological process this GO :0032757 and positive regulation of interleukin-8 production and biological process this GO :0034976 and response to endoplasmic reticulum stress and biological process this GO :0042594 and response to starvation and biological process this GO :0042789 and mRNA transcription from RNA polymerase II promoter and biological process this GO :0043161 and proteasome-mediated ubiquitin-dependent protein catabolic process and biological process this GO :0043433 and negative regulation of sequence-specific DNA binding transcription factor activity and biological process this GO :0043525 and positive regulation of neuron apoptotic process and biological process this GO :0043565 and sequence-specific DNA binding and molecular function this GO :0043620 and regulation of DNA-dependent transcription in response to stress and biological process this GO :0044267 and cellular protein metabolic process and biological process this GO :0044324 and regulation of transcription involved in anterior/posterior axis specification and biological process this GO :0045087 and innate immune response and biological process this GO :0045454 and cell redox homeostasis and biological process this GO :0045662 and negative regulation of myoblast differentiation and biological process this GO :0045892 and negative regulation of transcription, DNA-templated and biological process this GO :0045893 and positive regulation of transcription, DNA-templated and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0051209 and release of sequestered calcium ion into cytosol and biological process this GO :0070059 and intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress and biological process this GO :0090090 and negative regulation of canonical Wnt signaling pathway and biological process this GO :2000016 and negative regulation of determination of dorsal identity and biological process, Ddit3 and IDBG-194880 and ENSMUSG00000025408 and 13198, nuclei, sequence-specific DNA binding, this GO :0001955 and blood vessel maturation and biological process this GO :0003677 and DNA binding and molecular function this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0003714 and transcription corepressor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005770 and late endosome and cellular component this GO :0006355 and regulation of transcription, this GO :0003677 : DNA binding, this GO :0003677 : DNA binding and also this GO :0003700 : sequence-specific DNA binding transcription factor activity and also this GO :0003714 : transcription corepressor activity and also this GO :0005515 : protein binding and also this GO :0008134 : transcription factor binding and also this GO :0043565 : sequence-specific DNA binding, this GO :0003700 : sequence-specific DNA binding transcription factor activity, this GO :0003714 : transcription corepressor activity, this GO :0005515 : protein binding, this GO :0008134 : transcription factor binding, this GO :0043565 : sequence-specific DNA binding, DNA-damage-inducible transcript 3 |
| Identity: |
2726 |
| Gene: |
DDIT3 |
More about : DDIT3 |
| Long gene name: |
DNA damage inducible transcript 3 |
| Synonyms gene name: |
DNA-damage-inducible transcript 3 |
| Synonyms name: |
C/EBP zeta |
| Locus: |
12q13, 3 |
| Discovery year: |
1991-09-11 |
| GenBank acession: |
BC003637 |
| Entrez gene record: |
1649 |
| Pubmed identfication: |
1990262 |
| RefSeq identity: |
NM_004083 |
| Havana BLAST/BLAT: |
OTTHUMG00000170046 |