| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
DNAPK/PRKDC |
| Specificity: |
This antibody reacts specifically with DNAPK/PRKDC |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human DNA PKcs |
| Gene ID: |
5591 |
| Swiss Prot: |
P78527 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D, Also involved in modulation of transcription, Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA, Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 'Ser-588' and increasing CRY1 protein stability, DHX9, Found at the ends of chromosomes, HSPCA, Interacts with CRY1 and CRY2, Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination, MYC and RFA2, May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage, Must be bound to DNA to express its catalytic properties, PARP1, POU2F1, Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, Phosphorylates DCLRE1C, Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C), Recognizes the substrate consensus sequence [ST]-Q, Required to protect and align broken ends of DNA, SRF, The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step, WRN, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, c-Abl/ABL1, c-jun/JUN, histone H1, most likely through an indirect machanism, negatively regulates CRY1 phosphorylation, p53/TP53, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion, thereby regulating DNA damage response mechanism, Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
DNPK1 p350 DNAPK XRCC7 DNA-PKcs |
| Also known as: |
DNAPK/PRKDC Polyclonal Antibody |
| Other name: |
Anti-DNAPK/PRKDC Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
DNAPK/PRKDC |
| Short name: |
Anti-DNAPK/PRKDC (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, Desoxyribonucleic acid-activated, catalytic polypeptide (polyclonal), antibody to-DNAPK/protein kinase |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
DNA-PKcs and DNAPK and DNPK1 and HYRC and HYRC1 and IMD26 and p350 and XRCC7, DNA-activated, PRKDC and IDBG-408612 and ENSG00000253729 and 5591, PRKDC and IDBG-644289 and ENSBTAG00000017019 and 512740, Prkdc and IDBG-135771 and ENSMUSG00000022672 and 19090, alcohol group as acceptor, alcohol group as acceptor and also this GO :0019899 : enzyme binding and also this GO :0044822 : poly(A) RNA binding, alcohol group as acceptor and molecular function this GO :0018105 and peptidyl-serine phosphorylation and biological process this GO :0019899 and enzyme binding and molecular function this GO :0030098 and lymphocyte differentiation and biological process this GO :0031648 and protein destabilization and biological process this GO :0032481 and positive regulation of type I interferon production and biological process this GO :0032869 and cellular response to insulin stimulus and biological process this GO :0033077 and T cell differentiation in thymus and biological process this GO :0033152 and immunoglobulin V(D)J recombination and biological process this GO :0033153 and T cell receptor V(D)J recombination and biological process this GO :0035234 and ectopic germ cell programmed cell death and biological process this GO :0042752 and regulation of circadian rhythm and biological process this GO :0043065 and positive regulation of apoptotic process and biological process this GO :0044822 and poly(A) RNA binding and molecular function this GO :0045087 and innate immune response and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0048511 and rhythmic process and biological process this GO :0070419 and nonhomolo this GO us end joining complex and cellular component this GO :0072431 and signal transduction involved in mitotic G1 DNA damage checkpoint and biological process, catalytic polypeptide, nuclei, this GO :0000723 and telomere maintenance and biological process this GO :0001756 and somitogenesis and biological process this GO :0001933 and negative regulation of protein phosphorylation and biological process this GO :0002326 and B cell lineage commitment and biological process this GO :0002328 and pro-B cell differentiation and biological process this GO :0002360 and T cell lineage commitment and biological process this GO :0003677 and DNA binding and molecular function this GO :0004672 and protein kinase activity and molecular function this GO :0004674 and protein serine/threonine kinase activity and molecular function this GO :0004677 and DNA-dependent protein kinase activity and molecular function this GO :0005488 and binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005667 and transcription factor complex and cellular component this GO :0005730 and nucleolus and cellular component this GO :0005829 and cytosol and cellular component this GO :0005958 and DNA-dependent protein kinase-DNA ligase 4 complex and cellular component this GO :0006281 and DNA repair and biological process this GO :0006302 and double-strand break repair and biological process this GO :0006303 and double-strand break repair via nonhomolo this GO us end joining and biological process this GO :0006464 and cellular protein modification process and biological process this GO :0006468 and protein phosphorylation and biological process this GO :0007420 and brain development and biological process this GO :0007507 and heart development and biological process this GO :0008134 and transcription factor binding and molecular function this GO :0008630 and intrinsic apoptotic signaling pathway in response to DNA damage and biological process this GO :0010332 and response to gamma radiation and biological process this GO :0016020 and membrane and cellular component this GO :0016772 and transferase activity, this GO :0003677 : DNA binding, this GO :0003677 : DNA binding and also this GO :0004672 : protein kinase activity and also this GO :0004674 : protein serine/threonine kinase activity and also this GO :0004677 : DNA-dependent protein kinase activity and also this GO :0005488 : binding and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0008134 : transcription factor binding and also this GO :0016772 : transferase activity, this GO :0004672 : protein kinase activity, this GO :0004674 : protein serine/threonine kinase activity, this GO :0004677 : DNA-dependent protein kinase activity, this GO :0005488 : binding, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0008134 : transcription factor binding, this GO :0016772 : transferase activity, this GO :0016773 : phosphotransferase activity, this GO :0019899 : enzyme binding, this GO :0044822 : poly(A) RNA binding, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups and also this GO :0016773 : phosphotransferase activity, transferring phosphorus-containing groups and molecular function this GO :0016773 and phosphotransferase activity, protein kinase |
| Identity: |
9413 |
| Gene: |
PRKDC |
More about : PRKDC |
| Long gene name: |
DNA-activated, catalytic polypeptide , protein kinase |
| Synonyms gene: |
HYRC HYRC1 |
| Locus: |
8q11, 21 |
| Discovery year: |
1993-11-09 |
| Entrez gene record: |
5591 |
| Pubmed identfication: |
7638222 |
| RefSeq identity: |
NM_001081640 |
| Havana BLAST/BLAT: |
OTTHUMG00000164239 |
| Locus Specific Databases: |
LRG_162 |