| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
GPR40 |
| Specificity: |
This antibody reacts specifically with GPR40 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Extracellular |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human GPR40 |
| Gene ID: |
2864 |
| Swiss Prot: |
O14842 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Fatty acid binding increases glucose-stimulated insulin secretion, Ligand binding leads to a conformation change that triggers signaling via G-proteins that activate phospholipase C, May also play a role in bone homeostasis, Seems to act through a G(q) and G(i)-mediated pathway, and may also enhance the secretion of glucagon-like peptide 1 (GLP-1), leading to an increase of the intracellular calcium concentration, receptor signaling activates pathways that inhibit osteoclast differentiation (By similarity), G-protein coupled receptor for medium and long chain saturated and unsaturated fatty acids that plays an important role in glucose homeostasis |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
FFA1R |
| Also known as: |
GPR40 Polyclonal Antibody |
| Other name: |
Anti-GPR40 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
GPR40 |
| Short name: |
Anti-GPR40 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-GPR40 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
4498 |
| Gene: |
FFAR1 |
More about : FFAR1 |
| Long gene name: |
free fatty acid receptor 1 |
| Synonyms gene: |
GPR40 |
| Synonyms gene name: |
G protein-coupled receptor 40 |
| Locus: |
19q13, 12 |
| Discovery year: |
1997-10-10 |
| GenBank acession: |
AF024687 |
| Entrez gene record: |
2864 |
| Pubmed identfication: |
15684720 |
| RefSeq identity: |
NM_005303 |
| Classification: |
Free fatty acid receptors |
| Havana BLAST/BLAT: |
OTTHUMG00000188261 |