| Antigen detectable with this ELISA kit: |
Mast Cell (CMA1), Chymase 1 |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
125pg/mL, 15, 250pg/mL, 31, 500pg/mL, 15, 62, 25pg/mL, 5pg/mL, 625-1000pg/mL The standard curve concentrations used for the ELISA&rsquo, 625pg/mL, s were 1000pg/mL |
| Sensitivity: |
0pg/mL, The minimum detectable dose of this kit is typically less than 6 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Mast Cell (CMA1), Mast Cell (CMA1), Mast Cell (CMA1), Mast Cell (CMA1) in the samples is then determined by comparing the O, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chymase 1, The concentration of Chymase 1, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chymase 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Chymase 1, D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Mast (CMA1), Chymase 1 |
| Short name: |
Mast Cell (CMA1) ELISA kit, Chymase 1 |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Mast cellular (chymase 1, mast cell) Enzyme-linked immunosorbent assay reagent, Chymase 1 |
| Alternative technique: |
elisas |
| Alternative to gene target: |
CMA1 and IDBG-3997 and ENSG00000092009 and 1215, Cma1 and IDBG-166280 and ENSMUSG00000022225 and 17228, Extracellular, chymase and CYH and MCT1, mast cell, peptide binding, this GO :0002003 and angiotensin maturation and biological process this GO :0003824 and catalytic activity and molecular function this GO :0004252 and serine-type endopeptidase activity and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005622 and intracellular and cellular component this GO :0006508 and proteolysis and biological process this GO :0006518 and peptide metabolic process and biological process this GO :0008236 and serine-type peptidase activity and molecular function this GO :0022617 and extracellular matrix disassembly and biological process this GO :0030141 and secretory granule and cellular component this GO :0030198 and extracellular matrix organization and biological process this GO :0030901 and midbrain development and biological process this GO :0031012 and extracellular matrix and cellular component this GO :0042277 and peptide binding and molecular function this GO :0044267 and cellular protein metabolic process and biological process this GO :0045087 and innate immune response and biological process this GO :0045766 and positive regulation of angiogenesis and biological process this GO :0050720 and interleukin-1 beta biosynthetic process and biological process this GO :0050727 and regulation of inflammatory response and biological process this GO :0071333 and cellular response to glucose stimulus and biological process, this GO :0003824 : catalytic activity, this GO :0003824 : catalytic activity and also this GO :0004252 : serine-type endopeptidase activity and also this GO :0008236 : serine-type peptidase activity and also this GO :0042277 : peptide binding, this GO :0004252 : serine-type endopeptidase activity, this GO :0008236 : serine-type peptidase activity, this GO :0042277 : peptide binding, chymase 1 |
| Identity: |
2097 |
| Gene: |
CMA1 |
More about : CMA1 |
| Long gene name: |
chymase 1 |
| Synonyms gene name: |
chymase 1, mast cell |
| Locus: |
14q12 |
| Discovery year: |
1991-06-04 |
| Entrez gene record: |
1215 |
| Pubmed identfication: |
8468056 |
| RefSeq identity: |
NM_001836 |
| Havana BLAST/BLAT: |
OTTHUMG00000140181 |