| Antigen detectable with this ELISA kit: |
Chordin (CHRD) |
| Species reactivity: |
Rattus norvegicus (Rat) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
059ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma, tissue homogenates and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chordin (CHRD), The concentration of Chordin (CHRD) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chordin (CHRD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Chordin (CHRD), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Chordin (CHRD) |
| Short name: |
Chordin (CHRD) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Chordin (chordin) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
CHRD and IDBG-634990 and ENSBTAG00000002878 and 509942, CHRD and IDBG-68217 and ENSG00000090539 and 8646, Chrd and IDBG-146377 and ENSMUSG00000006958 and 12667, Extracellular, syndecan binding, this GO :0001501 and skeletal system development and biological process this GO :0001649 and osteoblast differentiation and biological process this GO :0001702 and gastrulation with mouth forming second and biological process this GO :0001707 and mesoderm formation and biological process this GO :0002053 and positive regulation of mesenchymal cell proliferation and biological process this GO :0005515 and protein binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0007275 and multicellular organismal development and biological process this GO :0007389 and pattern specification process and biological process this GO :0007417 and central nervous system development and biological process this GO :0008201 and heparin binding and molecular function this GO :0009953 and dorsal/ventral pattern formation and biological process this GO :0019955 and cytokine binding and molecular function this GO :0021919 and BMP signaling pathway involved in spinal cord dorsal/ventral patterning and biological process this GO :0030336 and negative regulation of cell migration and biological process this GO :0030514 and negative regulation of BMP signaling pathway and biological process this GO :0030900 and forebrain development and biological process this GO :0033504 and floor plate development and biological process this GO :0045545 and syndecan binding and molecular function this GO :0045668 and negative regulation of osteoblast differentiation and biological process this GO :0045785 and positive regulation of cell adhesion and biological process, this GO :0005515 : protein binding, this GO :0005515 : protein binding and also this GO :0008201 : heparin binding and also this GO :0019955 : cytokine binding and also this GO :0045545 : syndecan binding, this GO :0008201 : heparin binding, this GO :0019955 : cytokine binding, this GO :0045545 : syndecan binding, chordin |
| Identity: |
1949 |
| Gene: |
CHRD |
More about : CHRD |
| Long gene name: |
chordin |
| Locus: |
3q27, 1 |
| Discovery year: |
1998-11-06 |
| GenBank acession: |
AF076612 |
| Entrez gene record: |
8646 |
| Pubmed identfication: |
9782094 11472837 |
| RefSeq identity: |
NM_003741 |
| Havana BLAST/BLAT: |
OTTHUMG00000141267 |