| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
eNOS Ser633 |
| Specificity: |
This antibody reacts specifically with eNOS (Ser633) |
| Modification: |
Phosphorylation |
| Modification site: |
Ser633 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human eNOS around the phosphorylation site of Ser633 |
| Gene ID: |
4846 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
As with the other members of the NOS family, and cardiac myocyte function, and phosphorylation on numerous residues, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate, eNOS plays an important role in controlling vascular tone, including acylation with myristate and palmitate, platelet aggregation, eNOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
Constitutive NOS, EC NOS, Endothelial NOS, Endothelial nitric oxidase synthase, Endothelial nitric oxide synthase, Endothelial nitric oxide synthase 3, NOS 3, NOS III, NOS type III, NOS3, NOS3_HUMAN, NOSIII, Nitric oxide synthase 3, Nitric oxide synthase 3 endothelial cell, Nitric oxide synthase 3 endothelial cell, Nitric oxide synthase endothelial, cNOS, eNOS, eNOS 633, ecNOS, endothelial, nitric oxide synthase, p-eNOS phospho S633, eNOS phospho S633 |
| Also known as: |
eNOS (Ser633) Polyclonal Antibody |
| Other name: |
Anti-eNOS (Ser633) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
eNOS (Ser633) |
| Short name: |
Anti-eNOS (Ser633) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-eNOS (Ser633) (polyclonal) |
| Alternative technique: |
polyclonals |