| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
EEF2k Ser359 |
| Specificity: |
This antibody reacts specifically with EEF2k (Ser359) |
| Modification: |
Phosphorylation |
| Modification site: |
Ser359 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human EEF2k around the phosphorylation site of Ser359 |
| Gene ID: |
29904 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Phosphorylation of eEF2 by eEF2 kinase on specific threonine residues results in the inactivation of eEF-2 and in termination of mRNA translation, S6K1, The activity of eEF2 kinase is not only dependent upon Ca2+ ions, There is also evidence that eEF-2 phosphorylation is involved in the regulation of cell cycle progression, but is also regulated both negatively and positively via phosphorylation by different protein kinases (AMPK, calmodulin (CaM) and insulin, cellular differentiation, is an abundant cytoplasmic protein highly specific for elongation factor 2 (eEf2), oogensis and malignant tumors, p90 RSK), Eukaryotic elongation factor 2 kinase (EEF2k) previously known as Ca2+/calmodulin dependent protein kinase III |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
Calcium/calmodulin dependent eukaryotic elongation factor 2, Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase, Calmodulin dependent protein kinase III, EEF2K protein, EF2K_HUMAN, Elongation factor 2 kinase, Eukaryotic elongation factor 2 kinase, SMEF2K, cb365, eEF 2 kinase, eEF 2K, kinase eEF2K, p-EEF2K phospho S359, EEF2K phospho S359 |
| Also known as: |
EEF2k (Ser359) Polyclonal Antibody |
| Other name: |
Anti-EEF2k (Ser359) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
EEF2k (Ser359) |
| Short name: |
Anti-EEF2k (Ser359) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-EEF2k (Ser359) (polyclonal) |
| Alternative technique: |
polyclonals |