| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
EED |
| Specificity: |
This antibody reacts specifically with EED |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human EED |
| Gene ID: |
8726 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
5% identical to the mouse EED protein and contains seven WD repeats, 535 and 494 amino acids in length, EED (embryonic ectoderm development) is the human homolog of Eed, EED interacts in a highly specific manner, PcG proteins assemble into multimeric protein complexes, The human EED protein is 99, There are two human EED transcripts that contain a putative 407-nucleotide-long intron and give rise to two HEED protein isoforms, a murine PcG gene homologous to the Drosophila homeotic gene, both in vitro and in vivo, extra sex combs, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations, which are involved in protein-protein interactions, with histone deacetylase (HDAC) proteins, The transcriptional repressing Polycomb-group (PcG) and transcriptional activating trithorax-group (trxG) genes of Drosophila are part of a cellular memory system responsible for the stable inheritance of gene activity |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
WAIT-1 HEED |
| Also known as: |
EED Polyclonal Antibody |
| Other name: |
Anti-EED Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
EED |
| Short name: |
Anti-EED (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-EED (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
3188 |
| Gene: |
EED |
More about : EED |
| Long gene name: |
embryonic ectoderm development |
| Synonyms name: |
WD protein associating with integrin cytoplasmic tails 1 |
| Locus: |
11q14, 2 |
| Discovery year: |
1998-12-09 |
| GenBank acession: |
AF078933 |
| Entrez gene record: |
8726 |
| Pubmed identfication: |
9765275 9806832 |
| RefSeq identity: |
NM_003797 |
| Classification: |
Polycomb repressive complex 2 WD repeat domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000167209 |