| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
CSTF3/CSTF77 |
| Specificity: |
This antibody reacts specifically with CSTF3/CSTF77 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human CSTF3/CSTF77 |
| Gene ID: |
1479 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
77 kDa subunit), CstF-77 (cleavage stimulation factor, CstF-77 is capable of interacting with CPSF1 and FCP1, CstF-77 is highly conserved among eukaryotes, Heterotrimeric CstF recognizes GU- and U-rich sequences located downstream of the polyadenylation site on RNA, In addition, It can exist as a homodimer and functions as the bridge, It contains an Alpha-helical structure with 11 HAT (Half-a-TPR-containing) repeats and is essential for CstF assembly, The first step, a heterotrimer that is composed of three distinct subunits, also known as CstF3, directly interacting with the other two CstF subunits, endonucleolytic cleavage of polyadenylation substrates, is one of the three subunits comprising CstF, namely CstF-64 and CstF-50, other factors involved in polyadenylation, requires CstF (cleavage stimulation factor), Polyadenylation of mRNA precursors is a two-step reaction that requires multiple protein factors |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
CstF-77 |
| Also known as: |
CSTF3/CSTF77 Polyclonal Antibody |
| Other name: |
Anti-CSTF3/CSTF77 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
CSTF3/CSTF77 |
| Short name: |
Anti-CSTF3/CSTF77 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-CSTF3/CSTF77 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
2485 |
| Gene: |
CSTF3 |
More about : CSTF3 |
| Long gene name: |
cleavage stimulation factor subunit 3 |
| Synonyms gene name: |
3', 3', 77kD cleavage stimulation factor, cleavage stimulation factor, pre-RNA, pre-RNA, subunit 3, subunit 3 |
| Locus: |
11p13 |
| Discovery year: |
1995-01-16 |
| GenBank acession: |
U15782 |
| Entrez gene record: |
1479 |
| Pubmed identfication: |
7984242 |
| RefSeq identity: |
NM_001326 |
| Havana BLAST/BLAT: |
OTTHUMG00000166268 |