| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
BOB1 |
| Specificity: |
This antibody reacts specifically with BOB1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human BOB1 |
| Gene ID: |
5450 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
A novel protein designated Bob 1 (B cell Oct binding protein 1), ATTTGCAT, Although having no intrinsic capacity for DNA binding, Bob 1 associates tightly with the octamer motif in the presence of Oct-1 and/or Oct-2, Bob 1 is expressed at highest levels in spleen and peripheral blood leukocytes and represents an Oct co-factor capable of conferring cell-specific activation of Oct-1 and Oct-2, NF-A1, Oct-1 encodes one protein, Studies of tissue-specific expression of immunoglobulin promoters demonstrate the importance of an octamer, The POU dimer from the OCT1 gene formed on the palindromic OCT factor recognition element, The gene which encodes Bob 1 maps to human chromosome 11q23, The versatility of POU protein operation is additionally conferred at the dimerization level, This is a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes, alternatively called OBF-1, and the proteins that bind to it, could recruit the transcriptional coactivator OBF1, enhancing their transcriptional efficacy, or PORE (ATTTGAAATGCAAAT), specifically interacts with Oct-1 and Oct-2, which is encoded by Oct-2, which is found in nuclear extracts from all cell types and thus is not specific to lymphoid cells as is the protein NF-A2, 1, POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
OBF1 BOB1 |
| Also known as: |
BOB1 Polyclonal Antibody |
| Other name: |
Anti-BOB1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
BOB1 |
| Short name: |
Anti-BOB1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-BOB1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
9211 |
| Gene: |
POU2AF1 |
More about : POU2AF1 |
| Long gene name: |
POU class 2 associating factor 1 |
| Synonyms gene name: |
POU domain class 2, associating factor 1 |
| Locus: |
11q23, 1 |
| Discovery year: |
1995-11-08 |
| Entrez gene record: |
5450 |
| Pubmed identfication: |
8617501 |
| RefSeq identity: |
NM_006235 |
| Havana BLAST/BLAT: |
OTTHUMG00000166659 |