| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
XIAP/BIRC4 |
| Specificity: |
This antibody reacts specifically with XIAP |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from mouse XIAP |
| Gene ID: |
11798 |
| Swiss Prot: |
Q60989 |
| Applications: |
IF(ICC), IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(ICC)(1:50-200), IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
AIFM1, Acts as a direct caspase inhibitor, Acts as a positive regulator of Wnt signaling and ubiquitinates TLE1, Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and the target proteins for its E3 ubiquitin-protein ligase activity include: RIPK1, Acts as an important regulator of innate immune signaling via regulation of Nodlike receptors (NLRs), CASP3, CASP7, CASP8, CASP9, CCS and BIRC5/survivin, Can also function as E3 ubiquitin-protein ligase of the NEDD8 conjugation pathway, DIABLO/SMAC, Directly bind to the active site pocket of CASP3 and CASP7 and obstructs substrate entry, Inactivates CASP9 by keeping it in a monomeric, MAP3K2/MEKK2, Plays a role in copper homeostasis by ubiquitinationg COMMD1 and promoting its proteasomal degradation, Protects cells from spontaneous formation of the ripoptosome, Regulates the BMP signaling pathway and the SMAD and MAP3K7/TAK1 dependent pathways leading to NF-kappa-B and JNK activation, SOD1, Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8, TLE2, TLE3, TLE4 and AES, Ubiquitination of TLE3 results in inhibition of its interaction with TCF7L2/TCF4 thereby allowing efficient recruitment and binding of the transcriptional coactivator beta-catenin to TCF7L2/TCF4 that is required to initiate a Wnt-specific transcriptional program, Ubiquitinion of CCS leads to enhancement of its chaperone activity toward its physiologic target, Ubiquitinion of MAP3K2/MEKK2 and AIFM1 does not lead to proteasomal degradation, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner, as well as cell invasion and metastasis, but also modulates inflammatory signaling and immunity, cell proliferation, copper homeostasis, inactive state, mitogenic kinase signaling, rather than proteasomal degradation, targeting effector caspases for neddylation and inactivation, Multi-functional protein which regulates not only caspases and apoptosis |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
hILP |
| Also known as: |
XIAP Polyclonal Antibody |
| Other name: |
Anti-XIAP Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
XIAP |
| Short name: |
Anti-XIAP (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-X-linked inhibitor on apoptosis (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
XIAP and IDBG-85142 and ENSG00000101966 and 331, cysteine-type endopeptidase inhibitor activity involved in apoptotic process, nuclei, this GO :0004842 and ubiquitin-protein transferase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0006915 and apoptotic process and biological process this GO :0006974 and cellular response to DNA damage stimulus and biological process this GO :0008270 and zinc ion binding and molecular function this GO :0016055 and Wnt signaling pathway and biological process this GO :0016567 and protein ubiquitination and biological process this GO :0016874 and ligase activity and molecular function this GO :0030510 and regulation of BMP signaling pathway and biological process this GO :0031398 and positive regulation of protein ubiquitination and biological process this GO :0042127 and regulation of cell proliferation and biological process this GO :0043027 and cysteine-type endopeptidase inhibitor activity involved in apoptotic process and molecular function this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043154 and negative regulation of cysteine-type endopeptidase activity involved in apoptotic process and biological process this GO :0045087 and innate immune response and biological process this GO :0045088 and regulation of innate immune response and biological process this GO :0050727 and regulation of inflammatory response and biological process this GO :0055070 and copper ion homeostasis and biological process this GO :0070424 and regulation of nucleotide-binding oli this GO merization domain containing signaling pathway and biological process this GO :0090263 and positive regulation of canonical Wnt signaling pathway and biological process this GO :0097193 and intrinsic apoptotic signaling pathway and biological process this GO :1902530 and positive regulation of protein linear polyubiquitination and biological process, this GO :0004842 : ubiquitin-protein transferase activity, this GO :0004842 : ubiquitin-protein transferase activity and also this GO :0005515 : protein binding and also this GO :0008270 : zinc ion binding and also this GO :0016874 : ligase activity and also this GO :0043027 : cysteine-type endopeptidase inhibitor activity involved in apoptotic process, this GO :0005515 : protein binding, this GO :0008270 : zinc ion binding, this GO :0016874 : ligase activity, this GO :0043027 : cysteine-type endopeptidase inhibitor activity involved in apoptotic process, X-linked inhibitor of apoptosis |
| Identity: |
592 |
| Gene: |
XIAP |
More about : XIAP |
| Long gene name: |
X-linked inhibitor of apoptosis |
| Synonyms gene: |
API3 BIRC4 |
| Synonyms gene name: |
E3 ubiquitin protein ligase , baculoviral IAP repeat-containing 4 X-linked inhibitor of apoptosis |
| Locus: |
Xq25 |
| Discovery year: |
1998-06-10 |
| GenBank acession: |
U45880 |
| Entrez gene record: |
331 |
| Pubmed identfication: |
8552191 8654366 15749826 |
| RefSeq identity: |
NM_001167 |
| Classification: |
Baculoviral IAP repeat containing |
| Havana BLAST/BLAT: |
OTTHUMG00000022336 |
| Locus Specific Databases: |
BIRC4base: Mutation registry for X-linked lymphoproliferative syndrome Mental Retardation database LRG_19 |