| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
ARF1 |
| Specificity: |
This antibody reacts specifically with ARF1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human ARF1 |
| Gene ID: |
375 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, In its GTP-bound form, Involved in protein trafficking among different compartments, Modulates vesicle budding and uncoating within the Golgi complex, The hydrolysis of ARF1-bound GTP, an ADP-ribosyltransferase, is required for dissociation of coat proteins from Golgi membranes and vesicles, its triggers the association with coat proteins with the Golgi membrane, suggesting a crucial role in protein trafficking, which is mediated by ARFGAPs proteins, GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
ARF1 Polyclonal Antibody |
| Other name: |
Anti-ARF1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
ARF1 |
| Short name: |
Anti-ARF1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-ARF1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
652 |
| Gene: |
ARF1 |
More about : ARF1 |
| Long gene name: |
ADP ribosylation factor 1 |
| Locus: |
1q42, 13 |
| Discovery year: |
1992-07-09 |
| GenBank acession: |
M84326 |
| Entrez gene record: |
375 |
| Pubmed identfication: |
1577740 |
| RefSeq identity: |
NM_001024227 |
| Classification: |
ARF GTPase family Endogenous ligands |
| Havana BLAST/BLAT: |
OTTHUMG00000037595 |