| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
14-3-3 sigma |
| Specificity: |
This antibody reacts specifically with 14-3-3 sigma |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human 14-3-3 sigma |
| Gene ID: |
2810 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
14-3-3 s, 14-3-3 s acts to stimulate the Akt/mTOR signaling pathway by upregulating protein synthesis and cell growth, 14-3-3 s also functions to positively mediate IGF-I-induced cell cycle progression and can bind to a variety of translation initiation factors, 14-3-3 s is able to bind and modify the activity of a large number of proteins, 14-3-3 s positively regulates the tumor suppressor p53 and increases the rate of p53-regulated inhibition of G2/M cell cycle progression, Expressed predominately in stratified squamous keratinising epithelium, HME1 or YWHAS, In response to tumor growth, Multiple isoforms of 14-3-3 s exist due to alternative splicing events, Seven isoforms, When bound to Keratin 17, also known as SFN, comprise this family of signaling intermediates, denoted 14-3-3 b, e, for example, g, h, is a secreted adaptor protein that is involved in regulating both general and specific signaling pathways, mitogenic signaling and cell-cycle checkpoints, notably apoptosis, q and s, stratifin, such as KRT17 (Keratin 17), through recognition of a phosphothreonine or phosphoserine motif, thus controlling mitotic translation, z, 14-3-3 proteins regulate many cellular processes relevant to cancer biology |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
HS1 14-3-3 |
| Also known as: |
14-3-3 sigma Polyclonal Antibody |
| Other name: |
Anti-14-3-3 sigma Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
14-3-3 sigma |
| Short name: |
Anti-14-3-3 sigma (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-14-3-3 sigma (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
12854 |
| Gene: |
YWHAQ |
More about : YWHAQ |
| Long gene name: |
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta |
| Synonyms gene name: |
theta , theta polypeptide protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein |
| Synonyms name: |
protein tau |
| Locus: |
2p25, 1 |
| Discovery year: |
1999-08-20 |
| GenBank acession: |
AF070556 |
| Entrez gene record: |
10971 |
| Pubmed identfication: |
11080204 |
| RefSeq identity: |
NM_006826 |
| Classification: |
14-3-3 phospho-serine/phospho-threonine binding proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000013848 |