| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Prickle |
| Specificity: |
This antibody reacts specifically with Prickle |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Prickle |
| Gene ID: |
144165 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Defects in the gene encoding Prickle1 are associated with autosomal recessive progressive myoclonic epilepsy-1B, Expressed at higher levels in placenta and at lower levels in liver, Prickle1 is thought to function as a nuclear receptor that interacts with NRSF, The gene encoding Prickle1 maps to human chromosome 12, a silencer protein that binds the DNA sequence element NRSE (neuron-restrictive silencer element), as well as to the nuclear membrane, brain, kidney, lung and pancreas, shoulders or legs, which encodes over 1, which is characterized by quick jerks of the arms, 100 genes and comprises approximately 4, 5% of the human genome, Prickle1 is an 831 amino acid protein that contains one PET domain and three LIM zinc-binding domains and localizes to the cytoplasm |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
DKFZp686D143 |
| Also known as: |
Prickle Polyclonal Antibody |
| Other name: |
Anti-Prickle Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Prickle |
| Short name: |
Anti-Prickle (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Prickle (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
20340 |
| Gene: |
PRICKLE2 |
More about : PRICKLE2 |
| Long gene name: |
prickle planar cell polarity protein 2 |
| Synonyms gene name: |
prickle-like 2 (Drosophila) prickle homolog 2 (Drosophila) |
| Locus: |
3p14, 1 |
| Discovery year: |
2003-02-10 |
| GenBank acession: |
AK127839 |
| Entrez gene record: |
166336 |
| Pubmed identfication: |
12525887 |
| RefSeq identity: |
NM_198859 |
| Classification: |
Prickle planar cell polarity proteins LIM domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000158789 |