| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
EDA/Ectodermal dysplasia protein |
| Specificity: |
This antibody reacts specifically with EDA |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Extracellular |
| Antigen Source: |
membrane form/secreted form, KLH conjugated synthetic peptide derived from human Ectodysplasin-A |
| Gene ID: |
1896 |
| Applications: |
IF(IHC-P), FCM |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200), FCM(1:20-100) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
EDA is a member of the TNF-related ligand family involved in the early epithelial-mesenchymal interaction that regulates ectodermal appendage formation, EDA is a type II membrane protein which forms trimers, EDA is expressed in hair follicles and in the epidermis of adult skin, Ectodysplasin A (EDA) is a trimeric type II membrane protein that co-localizes with cytoskeletal structures at the lateral and apical surfaces of cells, Similar to other members of collagenous membrane proteins and members of TNF-related ligands, Some of the patients reported by Halperin and Curtis showed mental defect also, The sequence of the longest isoform includes an interrupted collagenous domain of 19 Gly-X-Y repeats and a motif conserved in the tumor necrosis factor (TNF)-related ligand family, abnormal teeth and absent sweat glands, but this is not an invariable feature, Affected males of X-linked anhidrotic ectodermal dysplasia show hypotrichosis |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
EDA1 XLHED HED XHED ED1-A1 ED1-A2 EDA-A1 EDA-A2 |
| Also known as: |
EDA Polyclonal Antibody |
| Other name: |
Anti-EDA Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
EDA |
| Short name: |
Anti-EDA (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-ectodysplasin A (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
ECTD1 and ED1 and ED1-A1 and ED1-A2 and EDA-A1 and EDA-A2 and EDA1 and EDA2 and HED and HED1 and ODT1 and STHAGX1 and XHED and XLHED, EDA and IDBG-636361 and ENSBTAG00000012543 and 616179, EDA and IDBG-74170 and ENSG00000158813 and 1896, Eda and IDBG-161929 and ENSMUSG00000059327 and 13607, Extracellular, protein binding, this GO :0001942 and hair follicle development and biological process this GO :0005102 and receptor binding and molecular function this GO :0005164 and tumor necrosis factor receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005581 and collagen trimer and cellular component this GO :0005789 and endoplasmic reticulum membrane and cellular component this GO :0005856 and cytoskeleton and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005887 and integral component of plasma membrane and cellular component this GO :0006955 and immune response and biological process this GO :0007160 and cell-matrix adhesion and biological process this GO :0007165 and signal transduction and biological process this GO :0007398 and ectoderm development and biological process this GO :0010467 and gene expression and biological process this GO :0016020 and membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0030154 and cell differentiation and biological process this GO :0042346 and positive regulation of NF-kappaB import into nucleus and biological process this GO :0042475 and odontogenesis of dentin-containing tooth and biological process this GO :0043123 and positive regulation of I-kappaB kinase/NF-kappaB signaling and biological process this GO :0043231 and intracellular membrane-bounded organelle and cellular component this GO :0043473 and pigmentation and biological process this GO :0043588 and skin development and biological process this GO :0045177 and apical part of cell and cellular component this GO :0051092 and positive regulation of NF-kappaB transcription factor activity and biological process this GO :0060662 and salivary gland cavitation and biological process this GO :0060789 and hair follicle placode formation and biological process this GO :0061153 and trachea gland development and biological process this GO :0090263 and positive regulation of canonical Wnt signaling pathway and biological process, this GO :0005102 : receptor binding, this GO :0005102 : receptor binding and also this GO :0005164 : tumor necrosis factor receptor binding and also this GO :0005515 : protein binding, this GO :0005164 : tumor necrosis factor receptor binding, this GO :0005515 : protein binding, ectodysplasin A |
| Identity: |
3157 |
| Gene: |
EDA |
More about : EDA |
| Long gene name: |
ectodysplasin A |
| Synonyms gene: |
ED1 EDA2 ODT1 |
| Synonyms gene name: |
anhidrotic oligodontia 1 , ectodermal dysplasia 1 |
| Locus: |
Xq13, 1 |
| Discovery year: |
2001-06-22 |
| GenBank acession: |
U59227 |
| Entrez gene record: |
1896 |
| Pubmed identfication: |
8696334 18657636 16583127 |
| RefSeq identity: |
NM_001399 |
| Classification: |
Tumor necrosis factor superfamily |
| Havana BLAST/BLAT: |
OTTHUMG00000021764 |
| Locus Specific Databases: |
Mental Retardation database |