| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
MAEA/EMP/HLC10 |
| Specificity: |
This antibody reacts specifically with EMP |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human EMP |
| Gene ID: |
10296 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Although the exact function of Emp is unknown, Considered to assist with cell division and nuclear architecture, Emp can form a complex with F-actin, Emp contains one CTLH domain and one LisH domain, Emp is localized with condensed chromatin at prophase, Emp is suggested to be involvement in erythroblast-macrophage cell attachment, Expressed as 5 alternatively spliced isoforms, and inhibiting apoptosis of erythroblasts, nuclear spindle poles at metaphase and in the contractile ring during telophase and cytokinesis, terminal maturation and enucleation of erythroid cells, which is involved regulating actin distribution in erythroblasts and macrophages, Emp is a 396 amino acid ubiquitously expressed adhesion protein |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
EMP GID9 |
| Also known as: |
EMP Polyclonal Antibody |
| Other name: |
Anti-EMP Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
EMP |
| Short name: |
Anti-EMP (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-EMP (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
13731 |
| Gene: |
MAEA |
More about : MAEA |
| Long gene name: |
macrophage erythroblast attacher |
| Synonyms name: |
FYV10 homolog (S, GID complex subunit 9, cerevisiae) |
| Locus: |
4p16, 3 |
| Discovery year: |
2000-11-07 |
| GenBank acession: |
AF084928 |
| Entrez gene record: |
10296 |
| Pubmed identfication: |
9763581 |
| RefSeq identity: |
NM_005882 |
| Havana BLAST/BLAT: |
OTTHUMG00000160169 |