| Category: |
Antibody |
| Concentration: |
pH7, with 0, 02% sodium azide, 3, 5 mg/ml in 1X TBS, 5% BSA (US sourced) and 0 |
| Form: |
Purified antibody |
| Conjugation: |
Biotinylated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
MIF [Biotin Conjugate] |
| Host animal: |
Goat (Capra aegagrus hircus) |
| Clonality: |
Polyclonal (goat origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA (peptide), WB |
| Recommended dilutions: |
1-0, 3ug/ml, ELISA (peptide) LOD: 1:128000, Western blot: 0 |
| Notes: |
<, A non-animal protein (NAP) blocker is recommended, Do not use a blocking agent or diluent that contains biotin (including milk and some BSA preparations) when testing with biotinylated antibodies, 1, Optimal dilution of the MIF antibody should be determined by the researcher, p> |
| Intented use: |
This MIF antibodyis to be used only for research purposes and not for diagnostics |
| Gene ID #: |
4282 |
| Purity: |
Antigen affinity |
| Description: |
Additional name(s) for this target protein: Macrophage migration inhibitory factor |
| Immunogen: |
Amino acids NAANVGWNNSTFA were used as the immunogen for this MIF antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot and store the MIF antibody at -20 deg |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
MIF [ Conjugate] |
| Short name: |
MIF Antibody [ Conjugate] |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Label: |
Biotin |
| Alternative name: |
macrophage migration inhibitory factor (glycosylation-inhibiting factor) (Antibody to) [biotinilated Conjugate] |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
Extracellular, GIF and GLIF and MMIF, MIF and IDBG-401910 and ENSG00000240972 and 4282, MIF and IDBG-637094 and ENSBTAG00000007375 and 280858, Mif and IDBG-163956 and ENSMUSG00000033307 and 17319, phenylpyruvate tautomerase activity, signal transduction by p53 class mediator and biological process this GO :0030890 and positive regulation of B cell proliferation and biological process this GO :0031666 and positive regulation of lipopolysaccharide-mediated signaling pathway and biological process this GO :0032269 and negative regulation of cellular protein metabolic process and biological process this GO :0033033 and negative regulation of myeloid cell apoptotic process and biological process this GO :0033138 and positive regulation of peptidyl-serine phosphorylation and biological process this GO :0042056 and chemoattractant activity and molecular function this GO :0042127 and regulation of cell proliferation and biological process this GO :0042327 and positive regulation of phosphorylation and biological process this GO :0043030 and regulation of macrophage activation and biological process this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043406 and positive regulation of MAP kinase activity and biological process this GO :0043518 and negative regulation of DNA damage response, signal transduction by p53 class mediator and biological process this GO :0045087 and innate immune response and biological process this GO :0048146 and positive regulation of fibroblast proliferation and biological process this GO :0050178 and phenylpyruvate tautomerase activity and molecular function this GO :0050715 and positive regulation of cytokine secretion and biological process this GO :0050731 and positive regulation of peptidyl-tyrosine phosphorylation and biological process this GO :0050918 and positive chemotaxis and biological process this GO :0061078 and positive regulation of prostaglandin secretion involved in immune response and biological process this GO :0061081 and positive regulation of myeloid leukocyte cytokine production involved in immune response and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0070207 and protein homotrimerization and biological process this GO :0070374 and positive regulation of ERK1 and ERK2 cascade and biological process this GO :0071157 and negative regulation of cell cycle arrest and biological process this GO :0090238 and positive regulation of arachidonic acid secretion and biological process this GO :0090344 and negative regulation of cell aging and biological process this GO :1902166 and negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator and biological process this GO :2000343 and positive regulation of chemokine (C-X-C motif) ligand 2 production and biological process, this GO :0001516 and prostaglandin biosynthetic process and biological process this GO :0001934 and positive regulation of protein phosphorylation and biological process this GO :0002906 and negative regulation of mature B cell apoptotic process and biological process this GO :0004167 and dopachrome isomerase activity and molecular function this GO :0005102 and receptor binding and molecular function this GO :0005125 and cytokine activity and molecular function this GO :0005126 and cytokine receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0006954 and inflammatory response and biological process this GO :0007166 and cell surface receptor signaling pathway and biological process this GO :0007569 and cell aging and biological process this GO :0008283 and cell proliferation and biological process this GO :0009986 and cell surface and cellular component this GO :0010629 and negative regulation of gene expression and biological process this GO :0010739 and positive regulation of protein kinase A signaling and biological process this GO :0019752 and carboxylic acid metabolic process and biological process this GO :0030330 and DNA damage response, this GO :0004167 : dopachrome isomerase activity, this GO :0004167 : dopachrome isomerase activity and also this GO :0005102 : receptor binding and also this GO :0005125 : cytokine activity and also this GO :0005126 : cytokine receptor binding and also this GO :0005515 : protein binding and also this GO :0042056 : chemoattractant activity and also this GO :0050178 : phenylpyruvate tautomerase activity, this GO :0005102 : receptor binding, this GO :0005125 : cytokine activity, this GO :0005126 : cytokine receptor binding, this GO :0005515 : protein binding, this GO :0042056 : chemoattractant activity, this GO :0050178 : phenylpyruvate tautomerase activity, macrophage migration inhibitory factor (glycosylation-inhibiting factor) |
| Identity: |
7097 |
| Gene: |
MIF |
More about : MIF |
| Long gene name: |
macrophage migration inhibitory factor (glycosylation-inhibiting factor) |
| Synonyms gene: |
GLIF |
| Synonyms: |
GIF |
| Locus: |
22q11, 23 |
| Discovery year: |
1993-10-15 |
| GenBank acession: |
M25639 |
| Entrez gene record: |
4282 |
| Pubmed identfication: |
7558020 2552447 |
| RefSeq identity: |
NM_002415 |
| Havana BLAST/BLAT: |
OTTHUMG00000150773 |