| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
PMFBP1 |
| Specificity: |
This antibody reacts specifically with PMFBP1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human PMFBP1 |
| Gene ID: |
83449 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Due to evidence that PMFBP1 may play a role in sperm tail morphology, Localized to the nucleus, PMF-1 contains a coiled-coil domain which interacts with the leucine-zipper domain of Nrf2, PMF-1 is also a component of the MIS12 complex, PMF-1 is expressed at highest levels in skeletal muscle and heart, PMFBP1 (Polyamine-modulated factor 1-binding protein 1) is a 1022 amino acid protein that binds PMF-1 and may be involved in general organization of the cytoskeleton, There are three isoforms of PMFBP1 that are produced as a result of alternative splicing events, This interaction regulates the transcription of SSAT, a regulatory enzyme for polyamine catabolism, it may therefore affect fertility, which is required for kinetochore formation and chromosomal alignment and segregation, with moderate expression in liver and kidney, PMF-1 is a 205 amino acid protein involved in kinetochore formation |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
PMFBP1 Polyclonal Antibody |
| Other name: |
Anti-PMFBP1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
PMFBP1 |
| Short name: |
Anti-PMFBP1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-PMFBP1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
17728 |
| Gene: |
PMFBP1 |
More about : PMFBP1 |
| Long gene name: |
polyamine modulated factor 1 binding protein 1 |
| Locus: |
16q22, 2 |
| Discovery year: |
2002-05-21 |
| GenBank acession: |
AF239683 |
| Entrez gene record: |
83449 |
| Pubmed identfication: |
11468771 |
| RefSeq identity: |
NM_031293 |
| Havana BLAST/BLAT: |
OTTHUMG00000167827 |