| Antigen detectable with this ELISA kit: |
Cathepsin A (CTSA) |
| Species reactivity: |
Mus musculus (Mouse) |
| Range of detection: |
100pg/mL, 12, 200pg/mL, 25pg/mL, 50pg/mL, 6, 25-400pg/mL The standard curve concentrations used for the ELISA&rsquo, 25pg/mL, 5pg/mL, 6, s were 400pg/mL |
| Sensitivity: |
74pg/mL, The minimum detectable dose of this kit is typically less than 2 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, plasma, tissue homogenates, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cathepsin A (CTSA), The concentration of Cathepsin A (CTSA) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cathepsin A (CTSA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Cathepsin A (CTSA), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Gene: |
CTSA |
More about : CTSA |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Cathepsin A (CTSA) |
| Short name: |
Cathepsin A (CTSA) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Cathepsin A (cathepsin A) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
CTSA and IDBG-642969 and ENSBTAG00000027337 and 518169, CTSA and IDBG-78417 and ENSG00000064601 and 5476, Ctsa and IDBG-212379 and ENSMUSG00000017760 and 19025, GLB2 and GSL and NGBE and PPCA and PPGB, enzyme activator activity, nuclei, this GO :0004180 and carboxypeptidase activity and molecular function this GO :0004185 and serine-type carboxypeptidase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005739 and mitochondrion and cellular component this GO :0005764 and lysosome and cellular component this GO :0005783 and endoplasmic reticulum and cellular component this GO :0006508 and proteolysis and biological process this GO :0006665 and sphin this GO lipid metabolic process and biological process this GO :0006687 and glycosphin this GO lipid metabolic process and biological process this GO :0006886 and intracellular protein transport and biological process this GO :0008047 and enzyme activator activity and molecular function this GO :0016020 and membrane and cellular component this GO :0043085 and positive regulation of catalytic activity and biological process this GO :0043202 and lysosomal lumen and cellular component this GO :0043231 and intracellular membrane-bounded organelle and cellular component this GO :0044281 and small molecule metabolic process and biological process this GO :0070062 and extracellular vesicular exosome and cellular component, this GO :0004180 : carboxypeptidase activity, this GO :0004180 : carboxypeptidase activity and also this GO :0004185 : serine-type carboxypeptidase activity and also this GO :0005515 : protein binding and also this GO :0008047 : enzyme activator activity, this GO :0004185 : serine-type carboxypeptidase activity, this GO :0005515 : protein binding, this GO :0008047 : enzyme activator activity, cathepsin A |
| Identity: |
9251 |
| Long gene name: |
cathepsin A |
| Synonyms gene: |
GSL PPGB |
| Synonyms gene name: |
protective protein for beta-galactosidase (galactosialidosis) |
| Synonyms name: |
carboxypeptidase C lysosomal protective protein carboxypeptidase-L carboxypeptidase Y-like kininase deamidase lysosomal carboxypeptidase A urinary kininase |
| Locus: |
20q13, 12 |
| Discovery year: |
1986-01-01 |
| GenBank acession: |
M22960 |
| Entrez gene record: |
5476 |
| Pubmed identfication: |
2071143 |
| RefSeq identity: |
NM_000308 |
| Classification: |
Cathepsins |
| Havana BLAST/BLAT: |
OTTHUMG00000033078 |