| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
CART |
| Specificity: |
This antibody reacts specifically with CART |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human CART |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
CART localizes to areas of the central and peripheral nervous systems and is involved in feeding behavior when injected centrally, Expression of CART in the rat hypothalamus is modulated by nutritional status, Injection of CART peptides into the ventral tegmental area produces psychostimulant-like effects, Its identification in cell bodies and central terminals of vagal afferent neurons additionally suggests a role in brainstem mechanisms of meal termination and satiety, The CART (cocaine- and amphetamine-regulated transcript) neuropeptide is an mRNA that changes in response to psychostimulant drug administration, and injection of synthetic CART peptide into the forebrain ventricular system suppresses food intake, indicating a possible role in hypothalamic control of energy homeostasis, The CART gene encodes for a protein which has an important role in the regulation of appetite and body weight |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
CART |
| Also known as: |
CART Polyclonal Antibody |
| Other name: |
Anti-CART Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
CART |
| Short name: |
Anti-CART (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-CART (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
24323 |
| Gene: |
CARTPT |
More about : CARTPT |
| Long gene name: |
CART prepropeptide |
| Synonyms name: |
cocaine and amphetamine regulated transcript |
| Locus: |
5q13, 2 |
| Discovery year: |
2006-10-04 |
| GenBank acession: |
U16826 |
| Entrez gene record: |
9607 |
| Pubmed identfication: |
9590691 8647455 |
| RefSeq identity: |
NM_004291 |
| Havana BLAST/BLAT: |
OTTHUMG00000131261 |