| Antigen detectable with this ELISA kit: |
Caspase 8 (CASP8) |
| Species reactivity: |
Mus musculus (Mouse) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
069ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Tissue homogenates and other biological fluids |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Caspase 8 (CASP8), The concentration of Caspase 8 (CASP8) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Caspase 8 (CASP8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Caspase 8 (CASP8), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Caspase 8 (CASP8) |
| Short name: |
Caspase 8 (CASP8) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
apoptosis-related cysteine peptidase) Enzyme-linked immunosorbent assay reagent, caspase 8 (caspase 8 |
| Alternative technique: |
elisas |
| Alternative to gene target: |
ALPS2B and CAP4 and Casp-8 and FLICE and MACH and MCH5, CASP8 and IDBG-643207 and ENSBTAG00000015718 and 507481, CASP8 and IDBG-78534 and ENSG00000064012 and 841, Casp8 and IDBG-157464 and ENSMUSG00000026029 and 12370, apoptosis-related cysteine peptidase, cysteine-type endopeptidase activity involved in apoptotic signaling pathway, leucine rich repeat containing receptor signaling pathway and biological process this GO :0035877 and death effector domain binding and molecular function this GO :0036462 and TRAIL-activated apoptotic signaling pathway and biological process this GO :0042110 and T cell activation and biological process this GO :0042113 and B cell activation and biological process this GO :0042981 and regulation of apoptotic process and biological process this GO :0043005 and neuron projection and cellular component this GO :0043123 and positive regulation of I-kappaB kinase/NF-kappaB signaling and biological process this GO :0043124 and negative regulation of I-kappaB kinase/NF-kappaB signaling and biological process this GO :0043234 and protein complex and cellular component this GO :0044297 and cell body and cellular component this GO :0045087 and innate immune response and biological process this GO :0045121 and membrane raft and cellular component this GO :0045471 and response to ethanol and biological process this GO :0045651 and positive regulation of macrophage differentiation and biological process this GO :0045862 and positive regulation of proteolysis and biological process this GO :0046677 and response to antibiotic and biological process this GO :0046982 and protein heterodimerization activity and molecular function this GO :0051291 and protein heterooli this GO merization and biological process this GO :0051603 and proteolysis involved in cellular protein catabolic process and biological process this GO :0060546 and negative regulation of necroptotic process and biological process this GO :0060715 and syncytiotrophoblast cell differentiation involved in labyrinthine layer development and biological process this GO :0070243 and regulation of thymocyte apoptotic process and biological process this GO :0070423 and nucleotide-binding oli this GO merization domain containing signaling pathway and biological process this GO :0071260 and cellular response to mechanical stimulus and biological process this GO :0071407 and cellular response to organic cyclic compound and biological process this GO :0097110 and scaffold protein binding and molecular function this GO :0097153 and cysteine-type endopeptidase activity involved in apoptotic process and molecular function this GO :0097190 and apoptotic signaling pathway and biological process this GO :0097191 and extrinsic apoptotic signaling pathway and biological process this GO :0097193 and intrinsic apoptotic signaling pathway and biological process this GO :0097194 and execution phase of apoptosis and biological process this GO :0097199 and cysteine-type endopeptidase activity involved in apoptotic signaling pathway and molecular function this GO :0097202 and activation of cysteine-type endopeptidase activity and biological process this GO :0097284 and hepatocyte apoptotic process and biological process this GO :0097342 and ripoptosome and cellular component this GO :1900740 and positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway and biological process this GO :2001233 and regulation of apoptotic signaling pathway and biological process this GO :2001238 and positive regulation of extrinsic apoptotic signaling pathway and biological process this GO :2001239 and regulation of extrinsic apoptotic signaling pathway in absence of ligand and biological process, nuclei, this GO :0001525 and angiogenesis and biological process this GO :0001841 and neural tube formation and biological process this GO :0002224 and toll-like receptor signaling pathway and biological process this GO :0002756 and MyD88-independent toll-like receptor signaling pathway and biological process this GO :0004175 and endopeptidase activity and molecular function this GO :0004197 and cysteine-type endopeptidase activity and molecular function this GO :0005123 and death receptor binding and molecular function this GO :0005164 and tumor necrosis factor receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005739 and mitochondrion and cellular component this GO :0005741 and mitochondrial outer membrane and cellular component this GO :0005815 and microtubule organizing center and cellular component this GO :0005829 and cytosol and cellular component this GO :0005856 and cytoskeleton and cellular component this GO :0006508 and proteolysis and biological process this GO :0006915 and apoptotic process and biological process this GO :0006919 and activation of cysteine-type endopeptidase activity involved in apoptotic process and biological process this GO :0006921 and cellular component disassembly involved in execution phase of apoptosis and biological process this GO :0007507 and heart development and biological process this GO :0008233 and peptidase activity and molecular function this GO :0008234 and cysteine-type peptidase activity and molecular function this GO :0008625 and extrinsic apoptotic signaling pathway via death domain receptors and biological process this GO :0009409 and response to cold and biological process this GO :0016032 and viral process and biological process this GO :0030101 and natural killer cell activation and biological process this GO :0030225 and macrophage differentiation and biological process this GO :0030690 and Noc1p-Noc2p complex and cellular component this GO :0031264 and death-inducing signaling complex and cellular component this GO :0031265 and CD95 death-inducing signaling complex and cellular component this GO :0031625 and ubiquitin protein ligase binding and molecular function this GO :0032025 and response to cobalt ion and biological process this GO :0032355 and response to estradiol and biological process this GO :0032403 and protein complex binding and molecular function this GO :0032496 and response to lipopolysaccharide and biological process this GO :0034138 and toll-like receptor 3 signaling pathway and biological process this GO :0034142 and toll-like receptor 4 signaling pathway and biological process this GO :0034612 and response to tumor necrosis factor and biological process this GO :0035666 and TRIF-dependent toll-like receptor signaling pathway and biological process this GO :0035872 and nucleotide-binding domain, this GO :0004175 : endopeptidase activity, this GO :0004175 : endopeptidase activity and also this GO :0004197 : cysteine-type endopeptidase activity and also this GO :0005123 : death receptor binding and also this GO :0005164 : tumor necrosis factor receptor binding and also this GO :0005515 : protein binding and also this GO :0008233 : peptidase activity and also this GO :0008234 : cysteine-type peptidase activity and also this GO :0031625 : ubiquitin protein ligase binding and also this GO :0032403 : protein complex binding and also this GO :0035877 : death effector domain binding and also this GO :0046982 : protein heterodimerization activity and also this GO :0097110 : scaffold protein binding and also this GO :0097153 : cysteine-type endopeptidase activity involved in apoptotic process and also this GO :0097199 : cysteine-type endopeptidase activity involved in apoptotic signaling pathway, this GO :0004197 : cysteine-type endopeptidase activity, this GO :0005123 : death receptor binding, this GO :0005164 : tumor necrosis factor receptor binding, this GO :0005515 : protein binding, this GO :0008233 : peptidase activity, this GO :0008234 : cysteine-type peptidase activity, this GO :0031625 : ubiquitin protein ligase binding, this GO :0032403 : protein complex binding, this GO :0035877 : death effector domain binding, this GO :0046982 : protein heterodimerization activity, this GO :0097110 : scaffold protein binding, this GO :0097153 : cysteine-type endopeptidase activity involved in apoptotic process, this GO :0097199 : cysteine-type endopeptidase activity involved in apoptotic signaling pathway, caspase 8 |
| Identity: |
1509 |
| Gene: |
CASP8 |
More about : CASP8 |
| Long gene name: |
caspase 8 |
| Synonyms gene name: |
apoptosis-related cysteine peptidase , apoptosis-related cysteine protease caspase 8, caspase 8 |
| Synonyms: |
MCH5 MACH FLICE Casp-8 |
| Locus: |
2q33, 1 |
| Discovery year: |
1996-11-11 |
| GenBank acession: |
U60520 |
| Entrez gene record: |
841 |
| Pubmed identfication: |
8681376 8681377 |
| RefSeq identity: |
NM_001228 |
| Classification: |
Caspases Death effector domain containing Ripoptosome Death inducing signaling complex |
| Havana BLAST/BLAT: |
OTTHUMG00000132821 |
| Locus Specific Databases: |
CASP8base: Mutation registry for Caspase 8 deficiency LRG_34 |