| Antigen detectable with this ELISA kit: |
Caspase 14 (CASP14) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
054ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Caspase 14 (CASP14), The concentration of Caspase 14 (CASP14) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Caspase 14 (CASP14), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Caspase 14 (CASP14), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Caspase 14 (CASP14) |
| Short name: |
Caspase 14 (CASP14) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
apoptosis-related cysteine peptidase) Enzyme-linked immunosorbent assay reagent, caspase 14 (caspase 14 |
| Alternative technique: |
elisas |
| Alternative to gene target: |
CASP14 and IDBG-34154 and ENSG00000105141 and 23581, CASP14 and IDBG-642581 and ENSBTAG00000021106 and 517658, Casp14 and IDBG-169485 and ENSMUSG00000005355 and 12365, apoptosis-related cysteine peptidase, cysteine-type peptidase activity, nuclei, this GO :0004197 and cysteine-type endopeptidase activity and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0006508 and proteolysis and biological process this GO :0006915 and apoptotic process and biological process this GO :0008234 and cysteine-type peptidase activity and molecular function this GO :0008544 and epidermis development and biological process this GO :0008630 and intrinsic apoptotic signaling pathway in response to DNA damage and biological process this GO :0031424 and keratinization and biological process this GO :0045095 and keratin filament and cellular component this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0070268 and cornification and biological process this GO :0097192 and extrinsic apoptotic signaling pathway in absence of ligand and biological process, this GO :0004197 : cysteine-type endopeptidase activity, this GO :0004197 : cysteine-type endopeptidase activity and also this GO :0008234 : cysteine-type peptidase activity, this GO :0008234 : cysteine-type peptidase activity, caspase 14 |
| Identity: |
1502 |
| Gene: |
CASP14 |
More about : CASP14 |
| Long gene name: |
caspase 14 |
| Synonyms gene name: |
apoptosis-related cysteine peptidase , apoptosis-related cysteine protease caspase 14, caspase 14 |
| Synonyms: |
MICE MGC119078 MGC119079 |
| Synonyms name: |
apoptosis-related cysteine protease |
| Locus: |
19p13, 12 |
| Discovery year: |
1998-11-09 |
| Entrez gene record: |
23581 |
| Pubmed identfication: |
10203698 9792675 15301553 |
| RefSeq identity: |
NM_012114 |
| Classification: |
Caspases |
| Havana BLAST/BLAT: |
OTTHUMG00000183205 |