| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
MAP3K4/MEK kinase-4 |
| Specificity: |
This antibody reacts specifically with MAP3K4/MEK kinase-4 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human MAP3K4/MEK kinase-4 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
ASK 1 (MEK kinase-5) and MAP3K6 (MEK kinase-6), ASK1 activates both MEK-4 and MEK-3/MEK-6 pathways, Evidence suggests that MEK-3 is preferentially activated by MEK kinase-3 and that MEK-4 is activated by both MEK kinase-2 and MEK kinase-3, JNK and p38, MAP3Ks or MEKKs) phosphorylate and thereby activate the MEKs (also called MAP kinase kinases or MKKs), MEK kinase-1, MEK kinase-1 has been shown to phosphorylate MEK-1 via Raf-independent pathway, MEK kinase-2, MEK kinase-3, MEK kinase-4, MEK kinase-4 has been shown to specifically activate the JNK path-way, MKKKs, Mos, Raf-B, The MEK kinases (also designated MAP kinase kinase kinases, The MEK kinases include Raf-1, These activated MEKs in turn phosphorylate and activate the MAP kinases, including ERK, Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli including growth factors |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
MTK1 MAPKKK4 KIAA0213 |
| Also known as: |
MAP3K4/MEK kinase-4 Polyclonal Antibody |
| Other name: |
Anti-MAP3K4/MEK kinase-4 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
MAP3K4/MEK kinase-4 |
| Short name: |
Anti-MAP3K4/MEK kinase-4 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-mitogen-activated protein phosphorylation catalyst kinase phosphorylation catalyst 4/MEK phosphorylation catalyst-4 (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
Cytoplasm, MAP3K4 and IDBG-635619 and ENSBTAG00000020079 and 511779, MAP3K4 and IDBG-98770 and ENSG00000085511 and 4216, MAPKKK4 and MEKK 4 and MEKK4 and MTK1 and PRO0412, Map3k4 and IDBG-136472 and ENSMUSG00000014426 and 26407, this GO :0000165 and MAPK cascade and biological process this GO :0000186 and activation of MAPKK activity and biological process this GO :0001890 and placenta development and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004674 and protein serine/threonine kinase activity and molecular function this GO :0004709 and MAP kinase kinase kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0010225 and response to UV-C and biological process this GO :0010468 and regulation of gene expression and biological process this GO :0016772 and transferase activity, this GO :0004672 : protein kinase activity, this GO :0004672 : protein kinase activity and also this GO :0004674 : protein serine/threonine kinase activity and also this GO :0004709 : MAP kinase kinase kinase activity and also this GO :0004713 : protein tyrosine kinase activity and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0016772 : transferase activity, this GO :0004674 : protein serine/threonine kinase activity, this GO :0004709 : MAP kinase kinase kinase activity, this GO :0004713 : protein tyrosine kinase activity, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0016772 : transferase activity, this GO :0046872 : metal ion binding, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups and also this GO :0046872 : metal ion binding, transferring phosphorus-containing groups and molecular function this GO :0019100 and male germ-line sex determination and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0043507 and positive regulation of JUN kinase activity and biological process this GO :0045087 and innate immune response and biological process this GO :0046872 and metal ion binding and molecular function this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0060718 and chorionic trophoblast cell differentiation and biological process this GO :1900745 and positive regulation of p38MAPK cascade and biological process, mitogen-activated protein kinase kinase kinase 4 |
| Identity: |
6856 |
| Gene: |
MAP3K4 |
More about : MAP3K4 |
| Long gene name: |
mitogen-activated protein kinase kinase kinase 4 |
| Synonyms gene: |
MEKK4 |
| Locus: |
6q26 |
| Discovery year: |
1997-11-14 |
| GenBank acession: |
AF002715 |
| Entrez gene record: |
4216 |
| Pubmed identfication: |
9305639 |
| Classification: |
Mitogen-activated protein kinase kinase kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000015968 |