| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
SPG7/Paraplegin |
| Specificity: |
This antibody reacts specifically with SPG7/Paraplegin |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Paraplegin |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Defects in the gene encoding Paraplegin are the cause of spastic paraplegia type 7 (SPG7), HSPs are degenerative spinal cord disorders that are characterized by muscle spasms, Localized to the mitochrondrial membrane and expressed throughout the body, Paraplegin is a multi-pass membrane protein that is thought to be involved in signal transduction and chaperone-like activities in the mitochrondria, Recent studies suggest that SPG7 may be a mitochondrial-based disease, These mitochondrial dysfunctions lead to axonal degeneration and impaired axonal transport, a form of autosomal recessive hereditary spastic paraplegia (AR-HSP), as mutations in the Paraplegin gene lead to ragged-red fibers, in some cases, incontinence, oxidase-negative fibers and intense succinate dehydrogenase-stained areas of the mitochrondria, stiffness in the legs and, thus causing the neurodegeneration seen in HSPs, Paraplegin is a 795 amino acid metalloprotease that is a member of the AAA protein family |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
CAR SPG5C |
| Also known as: |
SPG7/Paraplegin Polyclonal Antibody |
| Other name: |
Anti-SPG7/Paraplegin Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
SPG7/Paraplegin |
| Short name: |
Anti-SPG7/Paraplegin (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-SPG7/Paraplegin (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
11237 |
| Gene: |
SPG7 |
More about : SPG7 |
| Long gene name: |
SPG7, paraplegin matrix AAA peptidase subunit |
| Synonyms gene: |
CMAR |
| Synonyms gene name: |
cell matrix adhesion regulator spastic paraplegia 7 (pure and complicated autosomal recessive) |
| Synonyms name: |
paraplegin |
| Locus: |
16q24, 3 |
| Discovery year: |
1998-06-25 |
| GenBank acession: |
Y16610 |
| Entrez gene record: |
6687 |
| Pubmed identfication: |
9635427 9634528 |
| RefSeq identity: |
NM_003119 |
| Classification: |
AAA ATPases |
| Havana BLAST/BLAT: |
OTTHUMG00000138046 |
| Locus Specific Databases: |
ALSOD, the Amyotrophic Lateral Sclerosis Online Genetic Database |