| Antigen detectable with this ELISA kit: |
Mast Cell (CPA3), Carboxypeptidase A3 |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
125pg/mL, 15, 250pg/mL, 7, 31, 62, 25pg/mL, 5pg/mL, 625pg/mL, 7, 813-500pg/mL The standard curve concentrations used for the ELISA&rsquo, 813pg/mL, s were 500pg/mL |
| Sensitivity: |
6pg/mL, The minimum detectable dose of this kit is typically less than 3 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Mast Cell (CPA3), Mast Cell (CPA3), Mast Cell (CPA3), Mast Cell (CPA3) in the samples is then determined by comparing the O, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carboxypeptidase A3, The concentration of Carboxypeptidase A3, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carboxypeptidase A3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Carboxypeptidase A3, D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Mast (CPA3), Carboxypeptidase A3 |
| Short name: |
Mast Cell (CPA3) ELISA kit, Carboxypeptidase A3 |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Mast cellular (carboxypeptidase A3 (mast cell)) Enzyme-linked immunosorbent assay reagent, Carboxypeptidase A3 |
| Alternative technique: |
elisas |
| Alternative to gene target: |
CPA3 and IDBG-60396 and ENSG00000163751 and 1359, CPA3 and IDBG-637892 and ENSBTAG00000015008 and 528454, Cpa3 and IDBG-133537 and ENSMUSG00000001865 and 12873, Extracellular, MC-CPA, this GO :0002002 and regulation of angiotensin levels in blood and biological process this GO :0002003 and angiotensin maturation and biological process this GO :0004180 and carboxypeptidase activity and molecular function this GO :0004181 and metallocarboxypeptidase activity and molecular function this GO :0005576 and extracellular region and cellular component this GO :0006508 and proteolysis and biological process this GO :0008233 and peptidase activity and molecular function this GO :0008270 and zinc ion binding and molecular function this GO :0030133 and transport vesicle and cellular component this GO :0030141 and secretory granule and cellular component this GO :0044267 and cellular protein metabolic process and biological process, this GO :0004180 : carboxypeptidase activity, this GO :0004180 : carboxypeptidase activity and also this GO :0004181 : metallocarboxypeptidase activity and also this GO :0008233 : peptidase activity and also this GO :0008270 : zinc ion binding, this GO :0004181 : metallocarboxypeptidase activity, this GO :0008233 : peptidase activity, this GO :0008270 : zinc ion binding, zinc ion binding, carboxypeptidase A3 (mast cell) |
| Identity: |
2298 |
| Gene: |
CPA3 |
More about : CPA3 |
| Long gene name: |
carboxypeptidase A3 |
| Synonyms gene name: |
carboxypeptidase A3 (mast cell) |
| Synonyms name: |
mast cell carboxypeptidase A tissue carboxypeptidase A |
| Locus: |
3q24 |
| Discovery year: |
1990-06-13 |
| Entrez gene record: |
1359 |
| RefSeq identity: |
NM_001870 |
| Classification: |
Carboxypeptidases |
| Havana BLAST/BLAT: |
OTTHUMG00000159526 |