| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
OTX2 |
| Specificity: |
This antibody reacts specifically with OTX2 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human OTX2 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
A molecular mechanism depending on a precise threshold of OTX proteins is necessary for the correct positioning of the isthmic region and for anterior brain patterning, OTX1 and OTX2, OTX1 and OTX2 play an important role during early and later events required for proper brain development in that they are involved in the processes of induction, OTX1 is involved in corticogenesis, OTX2 is also required in the early specification of the neuroectoderm, The genes which encode OTX1 and OTX2 map to human chromosomes 2p13 and 14q21-q22, and both OTX1 and OTX2 co-operate in patterning the developing brain through a dosage-dependent mechanism, are two murine homologs of the Drosophila orthodenticle (OTD), for the correct anterior neural plate specification and organization of the primitive streak, respectively, sensory organ development and pituitary functions, show a limited amino acid sequence divergence, specification and regionalization of the brain, which is destined to become the fore-midbrain, while OTX2 is necessary earlier in development, Transcription factors |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
OTX2 Polyclonal Antibody |
| Other name: |
Anti-OTX2 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
OTX2 |
| Short name: |
Anti-OTX2 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-OTX2 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
8522 |
| Gene: |
OTX2 |
More about : OTX2 |
| Long gene name: |
orthodenticle homeobox 2 |
| Synonyms gene name: |
orthodenticle homolog 2 (Drosophila) |
| Locus: |
14q22, 3 |
| Discovery year: |
1994-02-08 |
| GenBank acession: |
AF298117 |
| Entrez gene record: |
5015 |
| Pubmed identfication: |
7959790 |
| RefSeq identity: |
, NM_021728 |
| Classification: |
PRD class homeoboxes and pseudogenes |
| Havana BLAST/BLAT: |
OTTHUMG00000152338 |
| Locus Specific Databases: |
LOVD LRG_718 |