| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
SEMA5B/Semaphorin 5B |
| Specificity: |
This antibody reacts specifically with SEMA5B/Semaphorin 5B |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human SEMA5B/Semaphorin 5B |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Localized to the membrane, Members of this family of proteins are approximately 750 amino acids in length (including signal sequences) and are defined by a conserved extracellular â, SEMA5B (semaphorin-5B), SEMA5B is a single-pass type III protein that functions to convey positive axonal guidance cues and can induce calpain-mediated cleavage of calcineurin, Secreted and cell-bound semaphorins chemically attract and repel the growth of neural axons, Three isoforms exist due to alternative splicing events, also known as SEMAG, blocks of conserved sequences and no obvious repeats, guiding the development of intricate networks of neural tissue, is a 1151 amino acid member of the semaphorin family, &euro, &euro, &oelig, Semaphorins are a family of cell surface and secreted proteins that are conserved from insects to humans, semaphorinâ, domain of approximately 500 amino acids containing 14-16 cysteines |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
SemG KIAA1445 FLJ10372 |
| Also known as: |
SEMA5B/Semaphorin 5B Polyclonal Antibody |
| Other name: |
Anti-SEMA5B/Semaphorin 5B Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
SEMA5B/Semaphorin 5B |
| Short name: |
Anti-SEMA5B/Semaphorin 5B (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-SEMA5B/Semaphorin 5B (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
10737 |
| Gene: |
SEMA5B |
More about : SEMA5B |
| Long gene name: |
semaphorin 5B |
| Synonyms gene: |
SEMAG |
| Synonyms gene name: |
(semaphorin) 5B , sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain |
| Locus: |
3q21, 1 |
| Discovery year: |
1999-06-25 |
| GenBank acession: |
AB040878 |
| Entrez gene record: |
54437 |
| Pubmed identfication: |
8817451 |
| RefSeq identity: |
NM_001031702 |
| Classification: |
Semaphorins |
| Havana BLAST/BLAT: |
OTTHUMG00000140392 |