| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
SEMA4B |
| Specificity: |
This antibody reacts specifically with SEMA4B |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Extracellular |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human SEMA4B |
| Applications: |
IF(IHC-P), FCM |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200), FCM(1:20-100) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Containing one Ig-like C2-type (immunoglobulin-like) domain, Members of this family of proteins are approximately 750 amino acids in length (including signal sequences) and are defined by a conserved extracellular â, SEMA4B (semaphorin-4B), SEMA4B is encoded by a gene located on human chromosome 15, Secreted and cell-bound semaphorins chemically attract and repel the growth of neural axons, The transmembrane semaphorins are characterized by an additional 80 amino acid transmembrane domain and an 80-110 amino acid cytoplasmic domain, a PSI domain and a single sema domain, also known as SemC or SEMAC, blocks of conserved sequences and no obvious repeats, guiding the development of intricate networks of neural tissue, is an 832 amino acid single-pass type I membrane protein that belongs to the semaphorin family and exists as two alternatively spliced isoforms, &euro, &euro, &oelig, Semaphorins are a family of cell surface and secreted proteins that are conserved from insects to humans, semaphorinâ, domain of approximately 500 amino acids containing 14-16 cysteines |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
SemC KIAA1745 MGC131831 |
| Also known as: |
SEMA4B Polyclonal Antibody |
| Other name: |
Anti-SEMA4B Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
SEMA4B |
| Short name: |
Anti-SEMA4B (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
(semaphorin) 4B (polyclonal), ALEXA Fluor 594, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, antibody to-sema domain |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
(semaphorin) 4B, Plasma membranes, SEMA4B and IDBG-29866 and ENSG00000185033 and 10509, SEMA4B and IDBG-629265 and ENSBTAG00000021273 and 510140, SEMAC and SemC, Sema4b and IDBG-194649 and ENSMUSG00000030539 and 20352, immunoglobulin domain (Ig), protein binding, this GO :0004872 and receptor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005886 and plasma membrane and cellular component this GO :0007275 and multicellular organismal development and biological process this GO :0007399 and nervous system development and biological process this GO :0016020 and membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0030154 and cell differentiation and biological process this GO :0045202 and synapse and cellular component, this GO :0004872 : receptor activity, this GO :0004872 : receptor activity and also this GO :0005515 : protein binding, this GO :0005515 : protein binding, transmembrane domain (TM) and short cytoplasmic domain, sema domain |
| Identity: |
10730 |
| Gene: |
SEMA4B |
More about : SEMA4B |
| Long gene name: |
semaphorin 4B |
| Synonyms gene: |
SEMAC |
| Synonyms gene name: |
(semaphorin) 4B , immunoglobulin domain (Ig), sema domain, transmembrane domain (TM) and short cytoplasmic domain |
| Locus: |
15q25 |
| Discovery year: |
1999-06-25 |
| GenBank acession: |
AB051532 |
| Entrez gene record: |
10509 |
| Pubmed identfication: |
7748561 |
| RefSeq identity: |
NM_198925 |
| Classification: |
Semaphorins |
| Havana BLAST/BLAT: |
OTTHUMG00000172096 |