| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
EEA1 |
| Specificity: |
This antibody reacts specifically with EEA1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human EEA1 |
| Gene ID: |
942 |
| Swiss Prot: |
P42081 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Isoform 2 interferes with the formation of CD86 clusters, May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, and thus acts as a negative regulator of T-cell activation, by binding CD28 or CTLA-4, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation, Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
ZFYVE2 |
| Also known as: |
EEA1 Polyclonal Antibody |
| Other name: |
Anti-EEA1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
EEA1 |
| Short name: |
Anti-EEA1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-EEA1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
3185 |
| Gene: |
EEA1 |
More about : EEA1 |
| Long gene name: |
early endosome antigen 1 |
| Synonyms gene name: |
162kD , early endosome antigen 1 |
| Locus: |
12q22 |
| Discovery year: |
1998-12-23 |
| GenBank acession: |
L40157 |
| Entrez gene record: |
8411 |
| Pubmed identfication: |
7768953 9697774 |
| RefSeq identity: |
NM_003566 |
| Classification: |
Zinc fingers FYVE-type |
| Havana BLAST/BLAT: |
OTTHUMG00000170110 |