| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
PCDH20 |
| Specificity: |
This antibody reacts specifically with PCDH20 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Extracellular |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human PCDH20 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Hypermethylation of PCDH20 is associated with worse prognosis and clinical outcome, Importantly, In non-small cell lung cancer cell lines, Protocadherin-20 (PCDH20), Through inactivation or overexpression, a homozygous loss of PCDH20 was identified through either deletion of one allele and methylation of the other or methylation of both alleles, also known as protocadherin-13, is a 924 amino acid protein containing 6 cadherin domains and potentially functioning as a calcium-dependent cell-adhesion protein, protocadherins are cadherin-like cell adhesion proteins that contain up to seven extracellular domains and are predominantly expressed in the nervous system, several protocadherins have been implicated in a variety of cancers, suggesting that PCDH20 may function as a tumor suppressor, the adhesion mechanism of protocadherins is distinct from classic cadherins, As a subfamily of the cadherin superfamily |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
PCDH13 FLJ22218 |
| Also known as: |
PCDH20 Polyclonal Antibody |
| Other name: |
Anti-PCDH20 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
PCDH20 |
| Short name: |
Anti-PCDH20 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-protocadherin 20 (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
PCDH20 and IDBG-36781 and ENSG00000197991 and 64881, PCDH20 and IDBG-628593 and ENSBTAG00000011862 and 616023, Pcdh20 and IDBG-183462 and ENSMUSG00000050505 and 219257, Plasma membranes, poly(A) RNA binding, this GO :0005509 and calcium ion binding and molecular function this GO :0005886 and plasma membrane and cellular component this GO :0007156 and homophilic cell adhesion and biological process this GO :0016020 and membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0044822 and poly(A) RNA binding and molecular function, this GO :0005509 : calcium ion binding, this GO :0005509 : calcium ion binding and also this GO :0044822 : poly(A) RNA binding, this GO :0044822 : poly(A) RNA binding, protocadherin 20 |
| Identity: |
14257 |
| Gene: |
PCDH20 |
More about : PCDH20 |
| Long gene name: |
protocadherin 20 |
| Locus: |
13q21, 2 |
| Discovery year: |
2000-12-14 |
| GenBank acession: |
AF169693 |
| Entrez gene record: |
64881 |
| RefSeq identity: |
NM_022843 |
| Classification: |
Non-clustered protocadherins |
| Havana BLAST/BLAT: |
OTTHUMG00000017012 |