| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
MST4 + MST3 + STK25 Thr178 + Thr190 + Thr174 |
| Specificity: |
This antibody reacts specifically with MST4/MST3/STK25(T178/T190/T174) |
| Modification: |
Phosphorylation |
| Modification site: |
Thr178 + Thr190 + Thr174 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human MST-3 around the phosphorylation site of Thr178 + Thr190 + Thr174 |
| Gene ID: |
27347 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Caspase-mediated cleavage of the regulatory domain of MST3 activates its intrinsic kinase activity and leads to nuclear translocation, Expression of COOH-terminal truncated MST3 in cells results in DNA fragmentation and induction of apoptosis, In mammals, In yeast, Interaction with Golgi matrix protein GOLGA2 results in autophosphorylation on Thr-178, It can inhibit cell migration in a fashion dependent on autophosphorylation and can regulate paxillin phosphorylation through tyrosine phosphatase PTP-PEST, It is pro apoptotic and is highly expressed in placenta, MST3 contains a conserved kinase domain at its NH(2)-terminus and a regulatory domain at its COOH-terminus, MST3 or Mammalian Sterile 20-like kinase 3 is a member of the germinal center kinase-III family, Mitogen activated protein kinase cascades have been conserved throughout evolution, STK25 functions upstream of the MAPK cascade, This may also be activated by C terminal cleavage, and peripheral blood leukocytes, possibly as a consequence of stabilization of dimer formation, these cascades allow responses to complex stimuli such as growth factors and inflammatory cytokines, thymus, Novel human Ste20-related kinase Mst4 is biologically active in the activation of MEK/ERK pathway and in mediating cell growth and transformation |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
SOK1 YSK1 |
| Also known as: |
MST4/MST3/STK25(T178/T190/T174) Antibody |
| Other name: |
Anti-MST4/MST3/STK25(T178/T190/T174) |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Gene target: |
MST4/MST3/STK25(T178/T190/T174) |
| Short name: |
Anti-MST4/MST3/STK25(T178/T190/T174) Fluor 594 |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Serine/threonine-protein kinase MST4/MST3/STK25(T178/T190/T174) |
| Alternative to gene target: |
2610018G03Rik and IDBG-144889 and ENSMUSG00000031112 and 70415, MST4 and IDBG-630889 and ENSBTAG00000019652 and 539445, MST4 and IDBG-86408 and ENSG00000134602 and 51765, nuclei, this GO :0000139 and this GO lgi membrane and cellular component this GO :0000287 and magnesium ion binding and molecular function this GO :0004672 and protein kinase activity and molecular function this GO :0004674 and protein serine/threonine kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005794 and this GO lgi apparatus and cellular component this GO :0005813 and centrosome and cellular component this GO :0005829 and cytosol and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0006915 and apoptotic process and biological process this GO :0006921 and cellular component disassembly involved in execution phase of apoptosis and biological process this GO :0016772 and transferase activity, this GO :0000287 : magnesium ion binding, this GO :0000287 : magnesium ion binding and also this GO :0004672 : protein kinase activity and also this GO :0004674 : protein serine/threonine kinase activity and also this GO :0004713 : protein tyrosine kinase activity and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0016772 : transferase activity, this GO :0004672 : protein kinase activity, this GO :0004674 : protein serine/threonine kinase activity, this GO :0004713 : protein tyrosine kinase activity, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0016772 : transferase activity, this GO :0042802 : identical protein binding, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups and also this GO :0042802 : identical protein binding, transferring phosphorus-containing groups and molecular function this GO :0023014 and signal transduction by phosphorylation and biological process this GO :0042802 and identical protein binding and molecular function this GO :0042981 and regulation of apoptotic process and biological process, Serine/threonine-protein kinase MST4 |
| Identity: |
11404 |
| Gene: |
STK25 |
More about : STK25 |
| Long gene name: |
serine/threonine kinase 25 |
| Synonyms gene name: |
serine/threonine kinase 25 (Ste20, yeast homolog) |
| Locus: |
2q37, 3 |
| Discovery year: |
2000-01-18 |
| GenBank acession: |
D63780 |
| Entrez gene record: |
10494 |
| Pubmed identfication: |
8887545 9160885 15037601 |
| RefSeq identity: |
NM_006374 |
| Classification: |
STRIPAK complex |
| Havana BLAST/BLAT: |
OTTHUMG00000133408 |