| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Nanog |
| Specificity: |
This antibody reacts specifically with Nanog |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus |
| Antigen Source: |
KLH conjugated synthetic peptide derived from mouse Nanog |
| Gene ID: |
71950 |
| Swiss Prot: |
Q80Z64 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Rat (Rattus norvegicus), Mouse (Mus musculus) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, Acts as a transcriptional activator or repressor, Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3', Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes, Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages, When overexpressed, leading to recruitment of the NuRD complex and subsequent repression of expression, promotes cells to enter into S phase and proliferation, Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
FLJ12581 FLJ40451 |
| Also known as: |
Nanog Polyclonal Antibody |
| Other name: |
Anti-Nanog Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Nanog |
| Short name: |
Anti-Nanog (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Nanog (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
20857 |
| Gene: |
NANOG |
More about : NANOG |
| Long gene name: |
Nanog homeobox |
| Locus: |
12p13, 31 |
| Discovery year: |
2003-09-10 |
| GenBank acession: |
AB093576 |
| Entrez gene record: |
79923 |
| Pubmed identfication: |
12787505 12787504 |
| RefSeq identity: |
NM_024865 |
| Classification: |
NKL subclass homeoboxes and pseudogenes |
| Havana BLAST/BLAT: |
OTTHUMG00000166019 |