| Antigen detectable with this ELISA kit: |
Brain Derived Neurotrophic Factor (BDNF) |
| Species reactivity: |
Rattus norvegicus (Rat) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
055ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell culture supernates and other biological fluids, cell lysates, plasma, tissue homogenates, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Brain Derived Neurotrophic Factor (BDNF), The concentration of Brain Derived Neurotrophic Factor (BDNF) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Brain Derived Neurotrophic Factor (BDNF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Brain Derived Neurotrophic Factor (BDNF), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Brain Derived Neurotrophic Factor (BDNF) |
| Short name: |
Brain Derived Neurotrophic Factor (BDNF) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Brain Derived Neurotrophic Factor (brain-derived neurotrophic factor) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
ANON2 and BULN2, BDNF and IDBG-36817 and ENSG00000176697 and 627, BDNF and IDBG-636102 and ENSBTAG00000008134 and 617701, Bdnf and IDBG-195506 and ENSMUSG00000048482 and 12064, Extracellular, GABAergic and biological process this GO :0033138 and positive regulation of peptidyl-serine phosphorylation and biological process this GO :0033189 and response to vitamin A and biological process this GO :0034612 and response to tumor necrosis factor and biological process this GO :0035864 and response to potassium ion and biological process this GO :0042490 and mechanoreceptor differentiation and biological process this GO :0042493 and response to drug and biological process this GO :0042596 and fear response and biological process this GO :0043025 and neuronal cell body and cellular component this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043195 and terminal bouton and cellular component this GO :0043204 and perikaryon and cellular component this GO :0043523 and regulation of neuron apoptotic process and biological process this GO :0043524 and negative regulation of neuron apoptotic process and biological process this GO :0045666 and positive regulation of neuron differentiation and biological process this GO :0045843 and negative regulation of striated muscle tissue development and biological process this GO :0046668 and regulation of retinal cell programmed cell death and biological process this GO :0048167 and regulation of synaptic plasticity and biological process this GO :0048169 and regulation of long-term neuronal synaptic plasticity and biological process this GO :0048170 and positive regulation of long-term neuronal synaptic plasticity and biological process this GO :0048172 and regulation of short-term neuronal synaptic plasticity and biological process this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0048675 and axon extension and biological process this GO :0048839 and inner ear development and biological process this GO :0050890 and cognition and biological process this GO :0051602 and response to electrical stimulus and biological process this GO :0051965 and positive regulation of synapse assembly and biological process this GO :0055093 and response to hyperoxia and biological process this GO :0060079 and regulation of excitatory postsynaptic membrane potential and biological process this GO :0060548 and negative regulation of cell death and biological process this GO :0061193 and taste bud development and biological process this GO :0071356 and cellular response to tumor necrosis factor and biological process this GO :0071874 and cellular response to norepinephrine stimulus and biological process this GO :0072347 and response to anesthetic and biological process this GO :0097484 and dendrite extension and biological process this GO :1901215 and negative regulation of neuron death and biological process this GO :1902065 and response to L-glutamate and biological process this GO :1990089 and response to nerve growth factor and biological process this GO :1990090 and cellular response to nerve growth factor stimulus and biological process this GO :1990138 and neuron projection extension and biological process, NADH to ubiquinone and biological process this GO :0007214 and gamma-aminobutyric acid signaling pathway and biological process this GO :0007399 and nervous system development and biological process this GO :0007406 and negative regulation of neuroblast proliferation and biological process this GO :0007411 and axon guidance and biological process this GO :0007412 and axon target recognition and biological process this GO :0007611 and learning or memory and biological process this GO :0007612 and learning and biological process this GO :0007623 and circadian rhythm and biological process this GO :0007631 and feeding behavior and biological process this GO :0008021 and synaptic vesicle and cellular component this GO :0008038 and neuron recognition and biological process this GO :0008083 and growth factor activity and molecular function this GO :0009416 and response to light stimulus and biological process this GO :0009642 and response to light intensity and biological process this GO :0009725 and response to hormone and biological process this GO :0010996 and response to auditory stimulus and biological process this GO :0014047 and glutamate secretion and biological process this GO :0014076 and response to fluoxetine and biological process this GO :0014823 and response to activity and biological process this GO :0016023 and cytoplasmic membrane-bounded vesicle and cellular component this GO :0016358 and dendrite development and biological process this GO :0019222 and regulation of metabolic process and biological process this GO :0021675 and nerve development and biological process this GO :0030061 and mitochondrial crista and cellular component this GO :0030424 and axon and cellular component this GO :0030425 and dendrite and cellular component this GO :0030516 and regulation of axon extension and biological process this GO :0031667 and response to nutrient levels and biological process this GO :0032094 and response to food and biological process this GO :0032229 and negative regulation of synaptic transmission, growth factor activity, this GO :0001657 and ureteric bud development and biological process this GO :0001662 and behavioral fear response and biological process this GO :0001666 and response to hypoxia and biological process this GO :0002544 and chronic inflammatory response and biological process this GO :0005102 and receptor binding and molecular function this GO :0005169 and neurotrophin TRKB receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0006120 and mitochondrial electron transport, this GO :0005102 : receptor binding, this GO :0005102 : receptor binding and also this GO :0005169 : neurotrophin TRKB receptor binding and also this GO :0005515 : protein binding and also this GO :0008083 : growth factor activity, this GO :0005169 : neurotrophin TRKB receptor binding, this GO :0005515 : protein binding, this GO :0008083 : growth factor activity, brain-derived neurotrophic factor |
| Identity: |
1033 |
| Gene: |
BDNF |
More about : BDNF |
| Long gene name: |
brain derived neurotrophic factor |
| Synonyms name: |
neurotrophin |
| Locus: |
11p14, 1 |
| Discovery year: |
1991-01-15 |
| GenBank acession: |
AB038670 |
| Entrez gene record: |
627 |
| Pubmed identfication: |
2236018 1889806 17942328 17493809 |
| RefSeq identity: |
NM_170735 |
| Classification: |
Endogenous ligands |
| Havana BLAST/BLAT: |
OTTHUMG00000178797 |